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首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >The pathology of tuberculosis lesions in allergic skin test (PPD tuberculin) positive cows and detection of tuberculosis agents with PCR in milk samples
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The pathology of tuberculosis lesions in allergic skin test (PPD tuberculin) positive cows and detection of tuberculosis agents with PCR in milk samples

机译：过敏性皮肤试验（PPD结核菌素）阳性奶牛的结核病病变病理和PCR检测牛奶样品中的结核病菌

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摘要

The aim of this study was to determine tuberculosis (Tb) agents in milk and the pathologic changes in the tissues and organs of 100 PPD Tb-positive cows that were intended for slaughter. Milk samples were taken before slaughtering and PCR analysis was conducted to detect the Tb agent. In postmortem examinations, the lungs, liver, mammary glands, spleen, kidneys, and heart were collected for macroscopic and histopathological examinations. Out of the 100 PPD positive cows, lesions of Tb were observed in 75 cows. In the microscopic examination, Tb was identified in the lungs (51 cases), liver (61 cases), and in both the liver and lungs (37 cases). Although mammary Tb was suspected in 32 cows in macroscopic examinations, in microscopic examinations these cases were evaluated as chronic pyogenic abscesses mastitis. No Tb agents were detected by PCR analysis in milk samples taken from the cows before slaughter. Therefore, periodical milk inspections are suggested for controlling agents in milk. In some of the Tb PPD positive animals (25 cases), no changes were detected; therefore, there is a need to improve tests with higher sensitivity for the antemortem diagnosis of Tb. Moreover, people involved in the livestock sector should be trained about Tb, hygiene rules, and disease-free herds.
机译：这项研究的目的是确定奶牛中的结核病（Tb）病原以及100只PPD Tb阳性母牛的屠宰组织和器官的病理变化。屠宰前先采集牛奶样品，并进行PCR分析以检测Tb剂。在死后检查中，收集肺，肝，乳腺，脾脏，肾脏和心脏进行宏观和组织病理学检查。在100头PPD阳性母牛中，在75头母牛中观察到Tb损伤。在显微镜检查中，在肺（51例），肝（61例）以及肝和肺（37例）中均发现了Tb。尽管在宏观检查中怀疑32头母牛患有乳腺Tb，但在微观检查中，这些病例被评估为慢性化脓性脓肿性乳腺炎。通过PCR分析在屠宰前从母牛身上采集的牛奶样品中没有检测到Tb病原。因此，建议定期检查牛奶中的控制剂。在一些Tb PPD阳性动物（25例）中，未检测到任何变化。因此，需要改进对Tb的死前诊断具有更高灵敏度的测试。此外，应对畜牧业相关人员进行结核病，卫生规定和无病牛群的培训。

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