...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Turkish Journal of Veterinary and Animal Sciences >The effect of combining vitamin E and C on the viability improvement of transfected ovine spermatogonial stem cells after cryopreservation and thawing
【24h】

The effect of combining vitamin E and C on the viability improvement of transfected ovine spermatogonial stem cells after cryopreservation and thawing

机译：维生素E和C联合使用对冻存和解冻后转染的绵羊精原干细胞存活力的影响

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The present study aimed to identify whether vitamin E and C and their combination in basal freezing medium improved the viability of postthaw transfected spermatogonial stem cells (SSCs). For this purpose, SSCs were harvested by enzymatic digestion twice. After enrichment and culture on Sertoli cell feeder layer, SSCs were characterized by using alkaline phosphatase staining and expression of oct-4 and c-kit genes as specific stem cell markers. As to the transfection of SSCs, different concentrations of DNA (0.2, 0.4, and 0.8 μg) and turbofect (0.4 and 0.8 μL) in 100 μL of medium were studied. The results showed that cryopreservation of SSCs in the presence of 25 μg/mL vitamin E and 10 μg/mL vitamin C could increase cell viability and expression of antiapoptotic genes. The best combination of DNA and turbofect for transfection of SSCs in 100 μL of medium was 0.8 μg and 0.4 μL, respectively. However, SSCs indicated lower transfection efficiency compared with Sertoli cells (~30% vs. 70%, respectively). Cryopreservation with the addition of vitamin E and C in the basal freezing medium could increase cell viability of transfected SSCs as well. The findings of this study also suggest the need for further research regarding improvement of the transfection efficiency of SSCs.
机译：本研究旨在确定维生素E和C及其在基础冷冻培养基中的组合是否能提高融化后转染的精原干细胞（SSCs）的活力。为此，通过酶消化两次收获SSC。在Sertoli细胞饲养层上富集和培养后，通过使用碱性磷酸酶染色以及oct-4和c-kit基因的表达作为特异性干细胞标记物来表征SSC。关于SSC的转染，研究了100μL培养基中不同浓度的DNA（0.2、0.4和0.8μg）和Turbofect（0.4和0.8μL）。结果表明，在25μg/ mL维生素E和10μg/ mL维生素C的存在下冷冻保存SSCs可以增加细胞活力和抗凋亡基因的表达。 DNA和涡轮转染在100μL培养基中转染SSC的最佳组合分别为0.8μg和0.4μL。然而，与Sertoli细胞相比，SSCs的转染效率较低（分别约为30％和70％）。在基础冷冻培养基中添加维生素E和C进行冷冻保存也可以提高转染的SSC的细胞活力。这项研究的发现还表明，需要进一步研究提高SSCs转染效率。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号