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首页> 外文期刊>Viruses >Non-Structural Protein NSm of Tomato Spotted Wilt Virus Is an Avirulence Factor Recognized by Resistance Genes of Tobacco and Tomato via Different Elicitor Active Sites
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Non-Structural Protein NSm of Tomato Spotted Wilt Virus Is an Avirulence Factor Recognized by Resistance Genes of Tobacco and Tomato via Different Elicitor Active Sites

机译：番茄斑萎病毒的非结构蛋白NSm是一种通过不同诱导子活性位点被烟草和番茄抗性基因识别的无毒因子。

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Tomato spotted wilt virus (TSWV) is one of the most destructive viral pathogens of plants. Recently, a single dominant gene conferring complete resistance to TSWV (RTSW) was identified in Nicotina alata and introgressed into cultivated tobacco ( N. tabacum ). However, whether the TSWV carries an avirulence (Avr) factor directed against RTSW remains obscure. In the present study, we identified the non-structural protein (NSm), the movement protein of TSWV, which is an RTSW-specific Avr factor, by using two different transient expression systems. Using amino acid (aa) substitution mutants, we demonstrated the ability to induce RTSW-mediated hypersensitive response (HR) of NSm is independent of its movement function. Moreover, key substitutions (C118Y and T120N), a 21-aa viral effector epitope, and different truncated versions of NSm, which are responsible for the recognition of the Sw-5b resistance gene of tomato, were tested for their ability to trigger HR to TSWV in tobacco. Together, our results demonstrated that RTSW-mediated resistance is triggered by NSm in the same way as by Sw-5b, however, via different elicitor active sites. Finally, an Avr gene-based diagnostic approach was established and used to determine the presence and effectiveness of resistance genes in tobacco.
机译：番茄斑萎病毒（TSWV）是植物中最具破坏性的病毒病原体之一。最近，在尼古丁烟草中发现了一个赋予对TSWV（RTSW）完全抗性的单一显性基因，并渗入栽培烟草（N. tabacum）中。但是，TSWV是否携带针对RTSW的无毒力（Avr）因子仍然不清楚。在本研究中，我们通过使用两个不同的瞬时表达系统，鉴定了非结构蛋白（NSm），即TSWV的运动蛋白，它是RTSW特异性的Avr因子。使用氨基酸（aa）替代突变体，我们证明了诱导RTSW介导的NSm超敏反应（HR）的能力与其运动功能无关。此外，还测试了关键替代（C118Y和T120N），21-aa病毒效应物表位以及负责截断番茄Sw-5b抗性基因识别的不同截短版本的NSm，它们具有触发HR的能力。烟草中的TSWV。在一起，我们的结果表明，RTSW介导的耐药性是由NSm触发的，与Sw-5b相同，但是是通过不同的激发子活性位点触发的。最后，建立了基于Avr基因的诊断方法，并用于确定烟草中抗性基因的存在和有效性。

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