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首页> 外文期刊>Veterinary World >9. Cloning and sequencing gB , gD , and gM genes to perform the genetic variability of bovine herpesvirus-1 from Indonesia
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9. Cloning and sequencing gB , gD , and gM genes to perform the genetic variability of bovine herpesvirus-1 from Indonesia

机译：9.克隆和测序gB，gD和gM基因以进行来自印度尼西亚的牛疱疹病毒1的遗传变异

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Aim: Previous research has shown that bovine herpesvirus-1 (BHV-1) in Indonesia was closely related to subtype-1 based on glycoprotein D genes. This study aimed to analyze the genetic variability of the BHV-1 isolated from the recent case in Indonesia not only based on gD but also other genes such as gB and gM and to study the homology and similarity of the sample to other BHV-1 isolated in other countries or regions. Materials and Methods: Samples were drawn from the tracheal organ in recent field case and prepared for DNA extraction. The gB, gD, and gM were amplified using nested polymerase chain reaction (nPCR) with our specifically designed primer pair and based on the specified bands of 350 bp gB, 325 bp gD, and 734 bp gM confirmed as BHV-1. The PCR product was ligated into pGEM-T and transformed into competent Escherichia coli. The purified plasmid was subsequently sequenced. Results: The virus sample isolated from the recent field case of infectious bovine rhinotracheitis (IBR) from Indonesia showed variability based on the gB, gD, and gM sequences. However, all of the genes had high similarity (98-100%) to BHV-1.2. Conclusion: The recent field case of IBR in Indonesia was similar to BHV-1.2.
机译：目的：先前的研究表明印度尼西亚的牛疱疹病毒1（BHV-1）与基于糖蛋白D基因的亚型1密切相关。这项研究旨在分析从印度尼西亚最近的病例分离的BHV-1的遗传变异性，不仅基于gD，而且还基于其他基因，例如gB和gM，并研究样品与其他分离的BHV-1的同源性和相似性在其他国家或地区。材料和方法：在最近的田间病例中从气管器官中提取样品，并准备进行DNA提取。使用巢式聚合酶链反应（nPCR）和我们专门设计的引物对，并根据确认为BHV-1的350 bp gB，325 bp gD和734 bp gM的指定条带，扩增gB，gD和gM。将PCR产物连接到pGEM-T中，并转化入感受态大肠杆菌中。随后对纯化的质粒进行测序。结果：从印度尼西亚的传染性牛鼻气管炎（IBR）的最新现场病例中分离出的病毒样本显示出基于gB，gD和gM序列的变异性。但是，所有基因均与BHV-1.2具有高度相似性（98-100％）。结论：印尼最近的IBR现场病例类似于BHV-1.2。

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