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首页> 外文期刊>World Journal of Preventive Medicine >Sublethal UVB Induces DNA Lesions and Pro-Apoptotic Gene Transcription in Human Keratinocytes: Attenuation by a Mixture of Plant Extracts
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Sublethal UVB Induces DNA Lesions and Pro-Apoptotic Gene Transcription in Human Keratinocytes: Attenuation by a Mixture of Plant Extracts

机译：亚致死性UVB诱导人角质形成细胞中的DNA损伤和促凋亡基因转录：植物提取物混合物的衰减。

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Ultraviolet B (UVB) radiation is a potent environmental factor in skin pathogenesis as it modulates oxidative burst, gene expression and proliferation/apoptosis of human normal keratinocytes. In the absence of cell apoptosis, sublethal UVB is shown to induce significant increase of mitochondrial and nuclear DNA lesions as quantified by increased apurinic/apyrimidinic sites together with transcriptomic modulation of pro-apoptosis/survival genes. These effects may be attenuated by cell pretreatment with extracts from three various plants: Papaver rhoeas, Passiflora incarnata and Anchusa arvensis or their mixture. Extracts variably decreased UVB-induced mitochondrial and nuclear DNA lesions in normal human keratincoytes. When mixed together, higher reduction of DNA lesions has been observed at lower extracts’ concentrations, compared to each extract alone which suggested their synergy. Furthermore, sublethal UVB-induced transcriptomic modulation was significantly prevented by cell preincubation with extract mixture through coordinated reduction of pro-apoptotic gene transcription while increasing pro-survival related transcripts. Finally, extract mixture protected keratinocytes against lethal UVB dose. This work reveals sublethal UVB-mediated lesions at both DNA and RNA levels in human keratinocytes and the ability of appropriate plant-derived sunscreen to reduce cell injury.
机译：紫外线B（UVB）辐射是皮肤发病机理中的有效环境因素，因为它调节人正常角质形成细胞的氧化爆发，基因表达和增殖/凋亡。在不存在细胞凋亡的情况下，亚致死性UVB被证明可诱导线粒体和核DNA损伤的显着增加，这可以通过增加的嘌呤/嘧啶酮位点以及促凋亡/生存基因的转录组调控来定量。通过用三种不同植物的提取物对细胞进行预处理，可以减弱这些作用：罂粟，西番莲和西洋菜或其混合物。提取物在正常人的角蛋白小囊中可变地减少了UVB诱导的线粒体和核DNA损伤。混合在一起后，与单独提取的每种提取物相比，在较低提取物的浓度下观察到的DNA损伤降低程度更高，这表明它们具有协同作用。此外，通过与提取混合物的细胞预孵育，通过协同减少促凋亡基因转录，同时增加促存活相关转录本，可以显着防止亚致死UVB诱导的转录组调控。最后，提取混合物可保护角质形成细胞免受致命的UVB剂量的伤害。这项工作揭示了人类角质形成细胞中DNA和RNA水平的UVB介导的亚致死性损伤以及适当的植物源防晒霜减少细胞损伤的能力。

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