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Divergência genética observada entre cultivares e híbridos de manjeric?o

机译：罗勒品种与杂种之间的遗传差异

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Basil is an aromatic herb that stands out for its economic importance. It is consumed in natura and used to obtain essential oil. The cultivation of this species in several regions of the world has allowed variations by natural crosses and euploidy, leading to the wide genetic variability found nowadays. Considering the importance of this species, we aimed to analyze the genetic diversity of 27 basil genotypes using ISSR molecular markers. Fourteen primers were employed for DNA amplification, resulting in 86% polymorphism. Based on the Jaccard’s dissimilarity index, the highest index (0.80) was observed between the individuals BAS001 and BAS012, while the lowest index (0.18) was detected between the genotypes BAS014 and BAS015. The genetic similarity among individuals was calculated, forming four distinct clusters. Most individuals (40.7%) were allocated in cluster I. The polymorphic information content (PIC) (0.89) indicated considerable levels of genetic diversity among genotypes. In this sense, the ISSR markers were efficient in the detection of polymorphisms between the accessions, suggesting the genetic variability of the collection. This result demonstrates the importance of the use of molecular markers and the advantages that this information provides to the breeding of the species.
机译：罗勒是一种芳香植物，以其经济重要性而著称。它在natura中消耗，并用于获得精油。该物种在世界上几个地区的种植都允许通过自然杂交和整倍体进行变异，从而导致当今广泛的遗传变异。考虑到该物种的重要性，我们旨在使用ISSR分子标记分析27种罗勒基因型的遗传多样性。使用十四个引物进行DNA扩增，导致86％的多态性。根据杰卡德（Jaccard）的相异指数，在个体BAS001和BAS012之间观察到最高指数（0.80），而在BAS014和BAS015基因型之间观察到最低指数（0.18）。计算个体之间的遗传相似性，形成四个不同的簇。大多数个体（40.7％）被分配在群集I中。多态信息含量（PIC）（0.89）表明基因型之间的遗传多样性水平相当高。从这个意义上说，ISSR标记可有效检测种质之间的多态性，表明该种的遗传变异性。该结果证明了使用分子标记的重要性以及该信息为物种繁殖提供的优势。

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