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Chemical composition and antimicrobial activity of essential oil of Salvia officinalis L. and Salvia virgata Jacq

机译:丹参和丹参精油的化学成分和抑菌活性

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Background & AimThe genus Salvia (commonly known as sage) is a broad genus belonging to the family Lamiaceae. This study is aimed at assessing the essential oil composition and antimicrobial activities of two Salvia species. Experimental The chemical composition of the essential oils of Salvia officinalis L. and Salvia virgata Jacq, cultivated in Estahban (Fars, South Iran), was studied by means of GC-MSanalyses. Antimicrobial activity was tested against a panel of microorganisms including one Gram-positive (Staphylococcus epidermidis PTCC NO. 1435) and one Gram-negative (Escherichia coli) and three fungal strain (Alternaria alternata PTCC NO. 5224, Penicillium funiculosum PTCC NO. 5301 and P. funiculosum PTCC NO. 5169) using the disk diffusion and agar-well diffusion methods and the Minimum Inhibitory Concentration (MIC) technique. Results In all, 57 compounds were identified, 42 for S. officinalis, accounting for 98.94% ofthe total oil, 29 for S. virgata (98.81%). The major components of S. officinalis essential oil were ?±-thujone (37.18%), 1,8-cineole (12.71%), ?2-thujone (9.10%) and the major components of S. virgata essential oil were caryophyllene oxide (30.23%), ?2-caryophyllene (22.63%), sabinene (11.82%). The antimicrobial activity of the total essential oil evaluated by the agar-well diffusion method, the results showed that the highest active against S. epidermidis and also the least active against E. coli. Inhibition of growth was tested by the disk diffusion method, the results showed that essential oil of S. officinalis and S. virgata were highest active against E. coli and P. funiculosum (PTCC NO. 5301), respectively. Also the least active against A. alternata. Recommended applications/industries The results showed thatS. officinalis oil had higher antimicrobial activity compare to S. virgata
机译:背景与目的丹参属(Salvia)(俗称鼠尾草)是属于唇形科的广泛属。这项研究旨在评估两种丹参属植物的精油成分和抗菌活性。实验通过气相色谱-质谱分析法研究了在埃斯塔班(伊朗南部法尔斯)种植的丹参和丹参精油的化学成分。测试了针对一组微生物的抗菌活性,这些微生物包括一种革兰氏阳性(表皮葡萄球菌PTCC NO.1435)和一种革兰氏阴性(大肠杆菌)和三种真菌菌株(Alternaria alternata PTCC NO.5224,青霉菌PTCC NO.5301和P. funiculosum PTCC NO。5169),采用圆盘扩散和琼脂井扩散方法以及最小抑制浓度(MIC)技术。结果共鉴定出57种化合物,其中厚朴链球菌42种,占总油分的98.94%,粗叶葡萄球菌29种,占98.81%。山茱。精油的主要成分是α-thujone(37.18%),1,8-桉树脑(12.71%),α2-thujone(9.10%),而virgata精油的主要成分是石竹烯氧化物。 (30.23%),α2-石竹烯(22.63%),sa烯(11.82%)。通过琼脂井扩散法评估总香精油的抗菌活性,结果表明,其对表皮葡萄球菌的活性最高,对大肠杆菌的活性最小。通过圆盘扩散法测试了对生长的抑制作用,结果表明,厚朴链霉菌和维氏葡萄球菌的精油分别具有最高的对大肠杆菌和杀真菌假单胞菌的活性(PTCC NO.5301)。也是对交链曲霉最不活跃的。推荐应用/行业结果表明。厚朴油比S. virgata具有更高的抗菌活性

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