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首页> 外文期刊>Der Pharma Chemica: journal for medicinal chemistry, pharmaceutical chemistry and computational chemistry >In Vitro Antioxidant and Antimicrobial Properties of Murraya Paniculata L. Extracts as well as Identification of Their Active Secondary Metabolites by HPLC-ESI-MS
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In Vitro Antioxidant and Antimicrobial Properties of Murraya Paniculata L. Extracts as well as Identification of Their Active Secondary Metabolites by HPLC-ESI-MS

机译：辣木提取物的体外抗氧化和抗菌性能及其活性次生代谢产物的HPLC-ESI-MS鉴定

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The aim of this study was to determine the total phenolic content, antioxidant and antimicrobial properties of the methanolic extract of Murraya Paniculata leaves and its derived fractions as well as characterization the active chemical composition of these extracts by High-Performance Liquid Chromatography/Electrospray Ionization Tandem Mass Spectrometry (HPLC-ESI-MS). The antioxidant activity was evaluated by using two methods; DPPH free radical scavenging activity and total antioxidant capacity. The identification of certain chemical constituents of M. paniculata methanolic extract, ethyl acetate and butanolic fractions using HPLC-ESI-MS were carried out. The obtained results revealed that the ethyl acetate fraction contains highest contents of phenolic content (607.88 ± 6.87 mg gallic acid eq./g ext.) and flavonoids content (41.53 ± 0.63 mg rutin eq./g ext.). Also, ethyl acetate fraction exhibited the most potent antioxidant and antimicrobial activities.
机译：这项研究的目的是确定Murraya Paniculata叶片及其提取级分的甲醇提取物的总酚含量，抗氧化剂和抗菌性能，并通过高效液相色谱/电喷雾串联测定这些提取物的活性化学成分。质谱（HPLC-ESI-MS）。抗氧化活性通过两种方法评价。 DPPH自由基清除活性和总抗氧化能力。利用HPLC-ESI-MS鉴定了山M药甲醇提取物，乙酸乙酯和丁醇馏分的某些化学成分。获得的结果表明，乙酸乙酯级分包含最高含量的酚类含量（607.88±6.87mg没食子酸当量/ g ext。）和类黄酮含量（41.53±0.63mg芦丁当量/ g ext。）。同样，乙酸乙酯部分显示出最有效的抗氧化剂和抗菌活性。

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