The analysis of the plasma vitamin D of the pre-school children

Mohamed Kadari; M. Kaid; B. Dahmani; K. Allali

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The analysis of the plasma vitamin D of the pre-school children

机译：学龄前儿童血浆维生素D的分析

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The analysis of the plasma vitamin D of the pre-school children in paediatric department, in The University Hospital (CHU) of Tlémcen (Algerian willaya is located in the North West), is carried out by reversed phase high performance liquid chromatography with UV detection. This method is characterized by direct injection of the supernatant obtained by treatment of 100 μL of plasma with the mixture solvent C2H5OH/CH3COOC2H5 (1: 1). The quantification of the concentrations is carried out by using Cholecalciferol as an external standard; elution is made ingredient mode by using mixture CH3OH/CH3CN (70: 30%) and 1.5 mL/mn flow rate. The percentage of deficiency found in this study is 2.63 %.

机译：采用反相高效液相色谱-紫外检测技术对特伦森大学医院（CHU）儿科学龄前儿童的血浆维生素D进行了分析。。该方法的特征在于，直接注入用混合溶剂C2H5OH / CH3COOC2H5（1：1）处理100μL血浆获得的上清液。通过使用胆钙化固醇作为外标进行浓度的定量。通过使用CH3OH / CH3CN（70：30％）混合物和1.5 mL / mn流速进行成分模式洗脱。在这项研究中发现的不足百分比为2.63％。

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《Der Pharma Chemica: journal for medicinal chemistry, pharmaceutical chemistry and computational chemistry》 |2014年第1期|共5页
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Mohamed Kadari; M. Kaid; B. Dahmani; K. Allali;
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The analysis of the plasma vitamin D of the pre-school children

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