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首页> 外文期刊>Der Pharma Chemica: journal for medicinal chemistry, pharmaceutical chemistry and computational chemistry >Validated Chiral High Performance Liquid Chromatographic Method for Enantiomeric Separation of Epichlorohydrin on Immobilized mylose Based Stationary Phase
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Validated Chiral High Performance Liquid Chromatographic Method for Enantiomeric Separation of Epichlorohydrin on Immobilized mylose Based Stationary Phase

机译：经验证的手性高效液相色谱方法，用于固定化基于乳糖的固定相上的环氧氯丙烷对映体分离

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摘要

Chiral Liquid Chromatographic (CLC) method has been established and authenticated for the enantiomeric resolution of epichlorohydrin. Chiral resolution was achieved on Chiralpak-IA immobilized amylose based stationary phase using an eluent consisting of n-hexane:2-propanol (100:2 v/v) and flowing through column at rate of 1.0 ml/min. The column effluent was examined by using UV detector at 205 nm. Resolution between S-(+)-Epichlorohydrin (SE) and R-(-)-Epichlorohydrin (RE) was found to be ≥ 5. The method was authenticated for the quantification of RE in SE. The detector response for RE was proportionate over the concentrations range of 3-40 μg/ml. The Limit of Detection (LOD) and Limit of Quantification (LOQ) for RE were 0.95 μg/ml and 3.2 μg/ml respectively. Average recovery of the RE was in the range of 96.20% to 101.99%. Analytical solutions of both enantiomers in diluent were found to be stable for a day. The proposed method was found to be precise, accurate and robust for quantitative analysis of RE in SE.
机译：已经建立了手性液相色谱（CLC）方法并验证了表氯醇的对映体拆分。使用由正己烷：2-丙醇（100：2 v / v）组成的洗脱液，以1.0 ml / min的流速流过Chiralpak-IA固定化直链淀粉的固定相，实现了手性拆分。通过使用205 nm的UV检测器检查柱流出物。发现S-（+）-表氯醇（SE）和R-（-）-表氯醇（RE）之间的分离度≥5。该方法经验证可用于SE中RE的定量。 RE的检测器响应在3-40μg/ ml的浓度范围内成比例。 RE的检出限（LOD）和定量限（LOQ）分别为0.95μg/ ml和3.2μg/ ml。稀土的平均回收率在96.20％至101.99％的范围内。发现两种对映异构体在稀释剂中的分析溶液稳定一天。发现所提出的方法对于SE中RE的定量分析是精确，准确和可靠的。

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