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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Indirect Organogenesis in Summer Squash (Cucurbita pepo L.)
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Indirect Organogenesis in Summer Squash (Cucurbita pepo L.)

机译：西葫芦（南瓜）的间接器官发生

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Efficient plant regeneration via organogenesis was established for 2 summer squash (Cucurbita pepo L.) cultivars, viz. Bulum and Rumbo, using hypocotyl and cotyledon derived calli. Seeds were surface sterilized in 0.1% HgCl2 for 5 min, and germinated in vitro in plant growth regulator free MS media. The maximum morphogenic callus induction rate (86%) was observed from a hypocotyl explant by culturing in MS medium supplemented with 2.5 mg l-1 2,4-D. Calli size and fresh weight increased substantially through subculturing. The highest percentage of shoot regeneration (85%) and highest mean number of shoots (6.89) per culture were obtained with 0.5 mg l-1 thidiazuron. Initiation of multiple shoots through organogenesis from the calli was histologically proven. Hypocotyl explants were more responsive than cotyledon explants in terms of callus induction and subsequent plant regeneration. Regenerated shoots were rooted in MS medium supplemented with 1.0 mg l-1 IBA. About 70% of regenerated plantlets survived and showed new branch development under ex vitro conditions.
机译：通过2个西葫芦（Cucurbita pepo L.）品种，通过器官发生建立了有效的植物再生。 Bulum和Rumbo，使用下胚轴和子叶衍生的愈伤组织。将种子在0.1％HgCl2中表面灭菌5分钟，然后在无植物生长调节剂的MS培养基中体外发芽。从下胚轴外植体中观察到最大形态发生愈伤组织诱导率（86％），方法是在补充有2.5 mg -1，2,4-D的MS培养基中培养。通过传代培养，愈伤组织的大小和鲜重显着增加。用0.5 mg l-1噻唑隆获得了最高的芽再生百分比（85％）和最高的平均芽数（6.89）。通过愈伤组织的器官发生引发多芽的组织学证实。在愈伤组织诱导和随后的植物再生方面，下胚轴外植体比子叶外植体更敏感。将再生的芽生根于补充有1.0mg 1-1 IBA的MS培养基中。大约70％的再生小苗在离体条件下存活并显示出新的分支发育。

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