Identification and characterization of hydrolytic enzymes from the midgut of the cotton bollworm, Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

EBRU ?ZGüR; MERAL YüCEL; HüSEY?N AVN? ?KTEM

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Identification and characterization of hydrolytic enzymes from the midgut of the cotton bollworm, Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

机译：棉铃虫Helicoverpa armigeraHübner（鳞翅目：夜蛾科）中肠中水解酶的鉴定和表征

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Midgut hydrolytic enzymes of Helicoverpa armigera Hübner (Lepidoptera: Noctuidae) were identified and partially characterized. Km, Vmax, optimum pH, and specific activity were determined for proteolytic enzymes and a -amylases. All hydrolytic enzyme activity had an optimum pH value in the alkaline pH range. We observed major serine protease activity, together with minor cysteine-like activity, the former being significantly inhibited by soybean trypsin inhibitor (SBTI) and aprotinin. Moreover, different degrees of inhibition were observed with synthetic protease inhibitors. Electrophoretic methods revealed 3 isozymes of a -amylases, of which 2 had higher molecular weight and were more active than the other. Inhibition of amylolytic activity was observed with wheat a -amylase inhibitor (WAAI), whereas partially purified maize, chickpea, and bean seed crude extracts did not exhibit inhibitory activity toward a -amylases. To the best of our knowledge this is the first report on the properties of a -amylases from Helicoverpa armigera and the effects of several plant-originated a-amylase inhibitors on them.

机译：鉴定并部分表征了棉铃虫Hübner（鳞翅目：夜蛾科）的中肠水解酶。测定蛋白水解酶和α-淀粉酶的Km，Vmax，最佳pH和比活性。在碱性pH范围内，所有水解酶活性均具有最佳pH值。我们观察到主要的丝氨酸蛋白酶活性，以及次要的半胱氨酸样活性，前者被大豆胰蛋白酶抑制剂（SBTI）和抑肽酶显着抑制。此外，用合成蛋白酶抑制剂观察到了不同程度的抑制作用。电泳方法揭示了3种α-淀粉酶同工酶，其中2种具有较高的分子量，并且比其他酶更具活性。用小麦α-淀粉酶抑制剂（WAAI）观察到了淀粉分解活性的抑制作用，而部分纯化的玉米，鹰嘴豆和豆种子粗提物对α-淀粉酶没有抑制活性。据我们所知，这是关于棉铃虫α-淀粉酶特性的首次报道，以及几种植物来源的α-淀粉酶抑制剂对其的影响。

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《Turkish Journal of Agriculture & Forestry》 |2009年第3期|共10页
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EBRU ?ZGüR; MERAL YüCEL; HüSEY?N AVN? ?KTEM;
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中图分类农业基础科学;
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1. Identification and characterization of hydrolytic enzymes from the midgut of the cotton bollworm, Helicoverpa armigera Hubner (Lepidoptera: Noctuidae) [J] . Ozgur Ebru, Yucel Meral, Oktem Hueseyin Avni Turkish Journal of Agriculture & Forestry . 2009,第3期

机译：棉铃虫中肠中肠水解酶的鉴定与表征（鳞翅目：夜蛾科）
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Identification and characterization of hydrolytic enzymes from the midgut of the cotton bollworm, Helicoverpa armigera Hübner (Lepidoptera: Noctuidae)

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