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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Determination of the Effects of Hazelnut Husk and Tea Waste Treatments on Urease Enzyme Activity and Its Kinetics in Soil
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Determination of the Effects of Hazelnut Husk and Tea Waste Treatments on Urease Enzyme Activity and Its Kinetics in Soil

机译：榛子壳和茶渣处理对土壤脲酶活性及其动力学的影响

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In this research, the effects of 5% treatment of hazelnut husk (HH) and tea production waste (TEW) to clay loam soil on urease enzyme activity and its kinetics were determined in a 30-day soil incubation experiment. For this purpose, kinetic parameters (Vmax, Km, and Vmax/Km,) were calculated by determining urease activity in organic wastes treated soils in different substrate concentrations (0%, 1%, 2%, 4%, 6%, 8%, 10%, and 12%), incubation periods (0, 1, 2, 3, 4, 5 and 6 h), and incubation temperatures (0, 10, 20, 30, 40, and 50 °C) at the end of the 30 days of the incubation. The results of the study showed that: a) Treatments of soil with hazelnut husk and tea waste increased urease activity in soil, b) the reaction velocity increased as substrate concentration increased, however this increase continued up to 8% substrate concentration level in control soil and 10% substrate concentration level in organic waste amendment soil, c) While the reaction velocity of control soil became constant at 10% substrate concentration level, it became constant at 12% substrate concentration level in organic waste amendment soil. In both control and soil treated with organic wastes, the highest reaction velocity in substrate concentrations was determined at the incubation temperature of 50 °C. The highest Vmax in control and soil treated with organic wastes (TEW and HH) was observed at 50 °C. The highest Km was observed at 40 °C in control and at 50 °C in TEW and HH treatments. The highest Vmax/Km was observed at 50 °C in control, at 30 °C in HH treatment, and at 40 °C in TEW treatment soils
机译：在这项研究中，在30天的土壤培养实验中，确定了5％的榛子壳（HH）和茶叶生产废料（TEW）对粘土壤土的处理对脲酶活性和动力学的影响。为此，通过确定不同底物浓度（0％，1％，2％，4％，6％，8％）下有机废物处理过的土壤中的脲酶活性，计算出动力学参数（Vmax，Km和Vmax / Km）。 ，10％和12％），潜伏期（0、1、2、3、4、5和6小时）以及潜伏温度（0、10、20、30、40和50°C）孵化30天的时间。研究结果表明：a）用榛子壳和茶渣处理土壤增加了土壤中的脲酶活性，b）反应速度随着底物浓度的增加而增加，但是在对照土壤中这种增加持续到8％的底物浓度水平在有机废物改良土壤中底物浓度水平为10％，c）对照土壤的反应速度恒定在10％底物浓度水平，而在有机废物改良土壤中则为12％底物浓度水平。在对照和用有机废物处理过的土壤中，在50°C的孵育温度下测定的底物浓度最高反应速度。在50°C时，观察到对照和有机废物（TEW和HH）处理过的土壤中的最高Vmax。在对照中在40°C和TEW和HH处理中在50°C观察到最高Km。在对照中50°C，HH处理中30°C和TEW处理土壤中40°C时观察到最高Vmax / Km

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