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首页> 外文期刊>Turkish Journal of Agriculture & Forestry >Determination of the effects of hazelnut husk and tea waste treatments on urease enzyme activity and its kinetics in soil
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Determination of the effects of hazelnut husk and tea waste treatments on urease enzyme activity and its kinetics in soil

机译：确定榛子果壳和茶渣处理对土壤中脲酶活性及其动力学的影响

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摘要

In this research, the effects of 5% treatment of hazelnut husk (HH) and tea production waste (TEW) to clay loam Soil on urease enzyme activity and its kinetics were determined in a 30-day soil incubation experiment. For this purpose, kinetic parameters (V-max, K-m, and V-max/K-m,) were calculated by determining urease activity in organic wastes treated soils in different substrate concentrations (0%, 1%, 2%, 4%, 6%, 8%, 10%, and 12%), incubation periods (0, 1, 2, 3, 4, 5 and 6 h), and incubation temperatures (0, 10, 20, 30, 40, and 50 degrees C) at the end of the 30 days of the incubation. The results of the study showed that: a) Treatments of soil with hazelnut husk and tea waste increased urease activity in soil, b) the reaction velocity increased as substrate concentration increased, however this increase continued up to 8% substrate concentration level in control soil and 10% substrate concentration level in organic waste amendment soil, c) While the reaction velocity of control soil became constant at 10% substrate concentration level, it became constant at 12% substrate concentration level in organic waste amendment soil. In both control and soil treated with organic wastes, the highest reaction velocity in substrate concentrations was determined at the incubation temperature of 50 degrees C. The highest V-max in control and soil treated with organic wastes (TEW and HH) was observed at 50 degrees C. The highest K-m was observed at 40 degrees C in control and at 50 degrees C in TEW and HH treatments. The highest V-max/K-m was observed at 50 degrees C in control, at 30 degrees C in HH treatment, and at 40 degrees C in TEW treatment soils.
机译：在这项研究中，在30天的土壤培养实验中确定了5％的榛子壳（HH）和茶生产废料（TEW）对粘土壤土的处理对脲酶活性和动力学的影响。为此，通过确定不同底物浓度（0％，1％，2％，4％，6）下有机废物处理过的土壤中的尿素酶活性，计算动力学参数（V-max，Km和V-max / Km）。 ％，8％，10％和12％），孵育时间（0、1、2、3、4、5和6小时）和孵育温度（0、10、20、30、40和50摄氏度） ）在孵化30天后结束。研究结果表明：a）用榛子壳和茶渣处理土壤增加了土壤中的脲酶活性，b）反应速度随着底物浓度的增加而增加，但是在对照土壤中这种增加持续到8％的底物浓度水平在有机废物改良土壤中底物浓度水平为10％，c）对照土壤的反应速度在10％底物浓度水平下保持恒定，而在有机废物改良土壤中的反应速度在12％底物浓度水平下恒定。在对照和用有机废物处理的土壤中，在50℃的孵育温度下测定的底物浓度最高反应速度。在对照和用有机废物（TEW和HH）处理的土壤中观察到最高的V-max在对照中在40摄氏度和TEW和HH处理中在50摄氏度观察到最高Km。在对照中50摄氏度，HH处理中30摄氏度和TEW处理土壤中40摄氏度观察到最高的V-max / K-m。

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