...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Drug Design, Development and Therapy >Salidroside ameliorates autophagy and activation of hepatic stellate cells in mice via NF-κB and TGF-β1/Smad3 pathways
【24h】

Salidroside ameliorates autophagy and activation of hepatic stellate cells in mice via NF-κB and TGF-β1/Smad3 pathways

机译：红景天苷通过NF-κB和TGF-β1/ Smad3途径改善小鼠自噬和肝星状细胞活化

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Purpose: Liver fibrosis is commonly seen and a necessary stage in chronic liver disease. The aim of this study was to explore the effect of salidroside on liver fibrosis in mice and its potential mechanisms. Materials and methods: Two mouse liver fibrosis models were established by intraperitoneal injection of carbon tetrachloride (CCl4) for 8?weeks and bile duct ligation for 14?days. Salidroside was injected intraperitoneally at doses of 10 and 20?mg/kg once a day. Gene and protein expression levels were determined by quantitative real-time polymerase chain reaction, enzyme-linked immunosorbent assay, Western blot, immunohistochemistry, and immunofluorescence. Results: Salidroside inhibited the production of extracellular matrix (ECM) and regulated the balance between MMP2 and TIMP1 and, therefore, alleviated liver fibrosis in the two fibrosis models. Salidroside reduced the production of transforming growth factor (TGF)-β1 in Kupffer cells and hepatic stellate cells (HSCs) via the nuclear factor-κB signaling pathway and, therefore, inhibited the activation of HSCs and autophagy by downregulation of the TGF-β1/Smad3 signaling pathway. Conclusion: Salidroside can effectively attenuate liver fibrosis by inhibiting the activation of HSCs in mice.
机译：目的：肝纤维化是慢性肝病的常见病，是必不可少的阶段。这项研究的目的是探讨红景天苷对小鼠肝纤维化的影响及其潜在机制。材料与方法：腹腔注射四氯化碳（CCl 4 ）8周，建立胆管结扎14天，建立了两种小鼠肝纤维化模型。每天一次腹膜内注射红景天苷，剂量为10和20？mg / kg。通过定量实时聚合酶链反应，酶联免疫吸附测定，蛋白质印迹，免疫组化和免疫荧光测定基因和蛋白质的表达水平。结果：红景天苷抑制细胞外基质（ECM）的产生并调节MMP2和TIMP1之间的平衡，因此减轻了两种纤维化模型中的肝纤维化。红景天苷通过核因子-κB信号通路减少了Kupffer细胞和肝星状细胞（HSC）中转化生长因子（TGF）-β1的产生，因此，通过下调TGF-β1/来抑制HSC的活化和自噬。 Smad3信号通路。结论：红景天苷可通过抑制小鼠HSCs的激活而有效减轻肝纤维化。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号