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In vitro propagation of tuberose (Polianthes tuberosa)

机译：晚香玉（Polianthes tuberosa）的体外繁殖

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A plant regeneration method has been developed forPolianthes tuberosa.Outer and inner scales of bulb were used as explants.Both explants were cultured on MurashigeandSkoog (1962) medium containing 3% sucrose with different concentrations of benzylaminopurine (BAP), naphthalene acetic acid (NAA) and thidiazuron (TDZ).Multiple shoots were induced directly from the scales of explants. The formation of shoots from outer scales showed a slight difference from inner ones. The optimal concentration of BAP for inducing shoot formation was found to be 3.0 mg/L. Regeneration frequency was highest (93%) when explants were cultured on this MS medium. Another growth regulator that played an important role in shoot formation was TDZ (18µM). Regenerated shoots transferred to MS medium supplemented with 2.5 mg/L BAP combined with 0.5 mg/L NAA and 0.1 mg/L kinetin increased multiple shooting rates. Also, roots were formed when media was supplemented with different concentrations of NAA. The rooting frequency was highest in MS medium with 1 mg/L NAA. Well developed mericlones were successfully shifted into green house for acclimatization.
机译：已开发出一种针对马铃薯块茎的植物再生方法，将鳞茎的内外鳞片用作外植体。两种外植体均在MurashigeandSkoog（1962）培养基中培养，该培养基中含有3％的蔗糖，不同浓度的苄氨基嘌呤（BAP），萘乙酸（NAA）直接从外植体的尺度上诱导出许多芽。外鳞的芽与内鳞的芽稍有差异。诱导芽形成的最佳BAP浓度为3.0 mg / L。当在该MS培养基上培养外植体时，再生频率最高（93％）。在芽形成中起重要作用的另一种生长调节剂是TDZ（18μM）。将再生芽转移到补充有2.5 mg / L BAP，0.5 mg / L NAA和0.1 mg / L动蛋白的MS培养基中，可以提高多次射击的速度。同样，当培养基中添加不同浓度的NAA时，也会形成根。在含1 mg / L NAA的MS培养基中，生根频率最高。发达的美洛酮已成功转移到温室中进行驯化。

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