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首页> 外文期刊>Dynamic Chiropractic >Antimicrobial activity and chemical composition of essential oils of Stachys lavandulifolia Vahl. from Mazandaran , Iran
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Antimicrobial activity and chemical composition of essential oils of Stachys lavandulifolia Vahl. from Mazandaran , Iran

机译:Stachys lavandulifolia Vahl精油的抗菌活性和化学成分。来自伊朗Mazandaran

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Stachys lavandulifoliavahl. or Chaye Koohi isa native plant, which has been used as an anxiolytic and sedative in Iranian folk medicine which belongs to the Lamiaceae family. In this study,S. lavandulifoliavahl. were collected from mountain of Baladeh area in Mazandaran province, Iran. Chemical constituents of essential oil ofS. lavandulifoliavahl. were determined. Aerial parts were subjected to hydrodistillation (HD) in a Clevenger – type apparatus until there was no significant increase in the volume of the oil collected (3 h). The yield of the oil was 0.69% (w/w). The essential oil was analyzed by gas chromatography (GC) and GC-mass spectrometry (GC-MS). Identification of the components was based on GC retention indices computer matching with Wiley GC-MS library, and by comparison of the fragmentation patterns of the mass spectra with those reported in the literature. 59 components were identified constituting more than 99.1% of the oil. Hexadecanoic acid (13.9%), α-pinene (13.7%), germacrene-D (8.9%), β-pinene (7.0%), myrcene (4.5%), β-phellandrene (5.7%),Z-ocimene (3.4%), spathulenol (3.5%), δ-cadinene (2.0%) and α-cadinol (2.6%) were major components inS. lavandulifoliavahl. oil. The oil was tested against two strains of bacteria (Gram-positive and Gram-negative).In vitroantimicrobial activity of essential oil ofS. lavandulifoliavahl. were investigated by disc diffusion method and the minimum inhibitory concentration (MIC) and also minimal bactericidal concentration (MBC) determination. The studied sample was active against gram-positive and Gram-negative microbial strains. The oil exhibited higher activities against the Gram-negative tested bacterial strain.
机译:水苏lavandulifoliavahl。或Chaye Koohi是一种本土植物,曾在属于伊朗科的伊朗民间医学中用作抗焦虑和镇静剂。在这项研究中,S。 lavandulifoliavahl。采自伊朗Mazandaran省Baladeh地区的山区。 S的精油的化学成分。 lavandulifoliavahl。被确定。空气部分在Clevenger中进行加氢蒸馏(HD)。直到收集的油量没有明显增加(3小时)。油的产率为0.69%(w / w)。通过气相色谱(GC)和GC-质谱(GC-MS)分析精油。组分的鉴定基于与Wiley GC-MS库匹配的GC保留指数计算机,并通过比较质谱的碎片图谱与文献中报道的碎片图谱。确定了59种成分,占油的99.1%以上。十六烷酸(13.9%),α-pine烯(13.7%),germ并丁二烯D(8.9%),β-ne烯(7.0%),月桂烯(4.5%),β--烯(5.7%),Z-奥美替尼(3.4%),spathulenol(3.5%),δ-cadinene(2.0%)和α-cadinol(2.6%)是S中的主要成分。 lavandulifoliavahl。油。该油针对两种细菌菌株(革兰氏阳性和革兰氏阴性)进行了测试。S精油的体外抗菌活性。 lavandulifoliavahl。通过圆盘扩散法和最小抑菌浓度(MIC)以及最小杀菌浓度(MBC)测定进行研究。研究样品对革兰氏阳性和革兰氏阴性微生物菌株具有活性。该油对经革兰氏阴性测试的细菌菌株表现出更高的活性。

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