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首页> 外文期刊>Drug Target Insights >Effects of Ascorbic Acid, Alpha-Tocopherol and Allopurinol on Ischemia-Reperfusion Injury in Rabbit Skeletal Muscle: An Experimental Study
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Effects of Ascorbic Acid, Alpha-Tocopherol and Allopurinol on Ischemia-Reperfusion Injury in Rabbit Skeletal Muscle: An Experimental Study

机译：抗坏血酸，α-生育酚和别嘌呤醇对兔骨骼肌缺血再灌注损伤的影响：实验研究

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Purpose: Ischemia reperfusion injury to skeletal muscle, following an acute arterial occlusion is important cause of morbidity and mortality. The aim of the present study was to determine and evaluate the effects of ascorbic acide, alpha-tocopherol and allopurinol on ischemia reperfusion injury in rabbit skeletal muscle.Methods: Forty-eight New Zealand white rabbits, all male, weighing between 2.5 to 3.0 (mean 2.8) kg, were used in the study. They were separated into four groups. Group I was the control group without any drugs. The other groups were treatment groups (groups II, III, and IV). Group II rabbits administrated 50 mg/kg ascorbic acide and 100 mg/kg alpha-tocopherol 3 days prior to ischemia, group III rabbits received 50 mg/kg allopurinol 2 days prior to ischemia, and group IV rabbits were administrated both 50 mg/kg ascorbic acide, 100 mg/kg alpha-tocopherol 3 days prior to ischemia and 50 mg/kg allopurinol 2 days prior to ischemia. Two hours ischemia and 2 hours reperfusion were underwent to the treatment groups. At the end of the reperfusion periods, muscle samples were taken from rectus femoris muscle for determination of superoxide dismutase, catalase and glutathione peroxidase activities as antioxidant enzymes, and malondialdehyde as an indicator of lipid peroxidation and xanthine oxidase levels as source hydroxyl radical. Besides, histopathological changes (edema, inflammation, ring formation and splitting formation) were evaluated in the muscle specimens.Results: In the treatment groups; superoxide dismutase (U/mgprotein), catalase (U/mgprotein), and glutathione peroxidise (U/mgprotein) levels increased, malondialdehyde (nmol/mgprotein) and xanthine oksidase (mU/mgprotein) levels decreased compared to control I (p < 0.05). Increase of superoxide dismutase, catalase, and glutathione peroxidase levels were the highest and decrease of malondialdehyde and xanthine oxidase levels were the highest in group IV compared to groups II and III, but no significant as statistically. Also amount of cellular injury in group II, III, and IV were lower than group I.Conclusions: Antioxidant medication may help lowering ischemia reperfusion injury. In our study, all drug medications are shown to be able to have an effective role for preventing ischemia reperfusion injury. Moreover, ascorbic acide + alphatocopherol + allopurinol group (group IV) may have a benefi cial effect to decrease the local and systemic damage due to ischemia-reperfusion injury.
机译：目的：急性动脉阻塞后，骨骼肌缺血再灌注损伤是发病率和死亡率的重要原因。本研究的目的是确定和评估抗坏血酸，α-生育酚和别嘌呤醇对兔骨骼肌缺血再灌注损伤的作用。方法：48只新西兰白兔，均为雄性，体重在2.5至3.0之间（平均2.8公斤）用于研究。他们分为四组。第一组是没有任何药物的对照组。其他组为治疗组（II，III和IV组）。 II组兔子在缺血前3天服用50 mg / kg抗坏血酸和100 mg / kgα-生育酚，III组兔子在缺血前2天服用50 mg / kg别嘌醇，IV组兔子都在50 mg / kg服用抗坏血酸，缺血前3天服用100 mg / kgα-生育酚，缺血前2天服用50 mg / kg别嘌呤醇。治疗组进行了2小时的缺血和2小时的再灌注。在再灌注期结束时，从股直肌采集肌肉样品，以确定超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶的活性作为抗氧化酶，丙二醛作为脂质过氧化的指标，黄嘌呤氧化酶水平作为源羟基。此外，评估了肌肉标本的组织病理学变化（水肿，炎症，环形成和裂痕形成）。与对照I相比，超氧化物歧化酶（U / mg蛋白），过氧化氢酶（U / mg蛋白）和谷胱甘肽过氧化物（U / mg蛋白）水平增加，丙二醛（nmol / mg蛋白）和黄嘌呤奥糖苷酶（mU / mg蛋白）水平降低（p <0.05 ）。与II组和III组相比，IV组中超氧化物歧化酶，过氧化氢酶和谷胱甘肽过氧化物酶的增加最高，丙二醛和黄嘌呤氧化酶的减少最高，但在统计学上无显着性。 II，III和IV组的细胞损伤量也低于I组。结论：抗氧化药物可能有助于降低缺血再灌注损伤。在我们的研究中，所有药物均被证明能够有效预防缺血再灌注损伤。此外，抗坏血酸+α-生育酚+别嘌呤醇组（IV组）可能有益于减少局部缺血和再灌注损伤所致的全身性损害。

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