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酵素反応を用いた匂いカメラによる手掌部のエタノールガスの可視化計測

机译:使用气味反应器通过气味照相机视觉测量手掌中的乙醇气体

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摘要

An imaging system for gaseous ethanol transpired from human palm skin, based on an enzymatic reaction, was assembled and validated. This system uses a highly sensitive camera that measures gaseous ethanol concentrations as intensities of chemiluminescence from luminol’s reaction induced by alcohol oxidase (AOD) and a luminol-hydrogen peroxide-horseradish peroxidase (HRP) system. Conversion of gaseous ethanol concentrations and distributions proceed on an enzyme-immobilized mesh substrate with luminol solution in a dark box. In order to visualize ethanol transpired from human palm skin, we improved the chemiluminescence sensitivity of the imaging system with a mixture of a high-purity luminol solution of luminol sodium salt HG solution and an enhancer of eosin Y solution. The detection limit of the ethanol concentration was 4.9 ppm. This highly sensitive imaging allows successful visualization of ethanol transpired from palm skin.
机译:组装并验证了基于酶促反应从人棕榈皮肤中蒸出的气态乙醇的成像系统。该系统使用高度灵敏的相机,可测量乙醇中的气态乙醇浓度,该浓度是由醇氧化酶(AOD)和鲁米诺-过氧化氢-辣根过氧化物酶(HRP)系统诱导的鲁米诺反应产生的化学发光强度。气态乙醇浓度和分布的转换是在暗盒中的带有鲁米诺溶液的酶固定的网状基质上进行的。为了可视化从人手掌皮肤散发出的乙醇,我们将高纯度鲁米诺钠盐HG溶液的鲁米诺溶液和曙红Y溶液增强剂的混合物提高了成像系统的化学发光灵敏度。乙醇浓度的检出限为4.9ppm。这种高度灵敏的成像技术可以成功地可视化从棕榈皮中蒸出的乙醇。

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