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首页> 外文期刊>Journal of animal and veterinary advances >Isolation and Identification of Haemophilus parasuis and Establishment of its Detection Method of Indirect ELISA
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Isolation and Identification of Haemophilus parasuis and Establishment of its Detection Method of Indirect ELISA

机译：副猪嗜血杆菌的分离鉴定及其间接ELISA检测方法的建立

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To provide the evidence for detection and epidemical investigation of Haemophilus parasuis by means of isolation and identification of Haemophilus parasuis and study on its detection method of ELISA. From the pathological specimens collected from several scaled-up pig farms, the strain of Haemophilus parasuis was isolated identified by means of observation of cultural characteristics and morphology and structure, Biochemical tests, Antibiotic Susceptibility tests, PCR identification and serovar identification. Subsequently, indirect ELISA Method of Haemophilus parasuis antibody for detection was studied by utilizing the isolated strain bacteria of treated with ultrasonication as the antigen with preparation of positive serum, optimization of concentration of the coating antigen, concentration of the secondary antibody and reaction time and Susceptibility test, Specificity test and Repeatability test. A strain of Haemophilus parasuis serovar 5, named as SCHPS5 which is sensitive to cephalosporin IV, cefotaxime, sulbactam, furaxone, chloromycetin. The detection sensitivity of the target antibody in indirect ELISA Method based on this isolate can reach over 1:10000 and this detection method has high specificity and high repeatability. This study has provided scientific evidence and a reliable antibody detection method for prevention and treatment of the disease resulting from Haemophilus parasuis.
机译：通过对副猪嗜血杆菌的分离鉴定，ELISA检测方法的研究，为副猪嗜血杆菌的检测和流行病学调查提供依据。从几个规模化养猪场收集的病理标本中，通过观察培养物特征，形态和结构，生化测试，抗生素敏感性测试，PCR鉴定和血清学鉴定，分离出副猪嗜血杆菌菌株。随后，以分离的经超声处理的菌株细菌为抗原，制备了阳性血清，优化了包被抗原的浓度，二抗的浓度，反应时间和药敏性，研究了副猪嗜血杆菌抗体的间接ELISA检测方法。测试，特异性测试和重复性测试。副猪嗜血杆菌5型菌株，命名为SCHPS5，其对头孢菌素IV，头孢噻肟，舒巴坦，呋喃酮，氯霉素均敏感。基于该分离株的间接ELISA法中靶标抗体的检测灵敏度可以达到1：10000以上，并且具有高特异性和高重复性。该研究为预防和治疗副猪嗜血杆菌引起的疾病提供了科学依据和可靠的抗体检测方法。

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