"ELISA indirect detection method" is used to detect the level of local anti naive antibody produced in a real infection, avoid causing significant economic loss to livestock, and find "false positive reaction", which shows the cross reaction of anti retroviral antibody. 1. In the diagnosis of bovine brucellosis serum and animal and water tank milk intestinal infection and post vaccine antibody, it is characterized by the use of local anthrax other than B. melitensis 16m strain, without any effective adhesive capacity for any purification treatment;It is used for sensitizing or antivirus treatment of plates with known concentration (1 microgram per well),Noting the positive and negative control of the Leo method to ensure that it is maintained and to prevent contamination and degradation of existing antibodies, And ensure that the density in these controls is stable to correctly interpret the indirect results. In addition, the process of gonorrhea which can be used in other diagnosis methods is also introduced.
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