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首页> 外文期刊>Journal of Analytical & Bioanalytical Techniques >Determination of Intramolecular 13C Isotope Distribution of Pyruvate byHeadspace Solid Phase Microextraction-Gas Chromatography-Pyrolysis-GasChromatography-Combustion- Isotope Ratio Mass Spectrometry (HS-SPMEGC-Py-GC-C-IRMS) Method
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Determination of Intramolecular 13C Isotope Distribution of Pyruvate byHeadspace Solid Phase Microextraction-Gas Chromatography-Pyrolysis-GasChromatography-Combustion- Isotope Ratio Mass Spectrometry (HS-SPMEGC-Py-GC-C-IRMS) Method

机译：顶空固相微萃取-气相色谱-热解-气相色谱-燃烧-同位素比质谱（HS-SPMEGC-Py-GC-C-IRMS）方法测定丙酮酸的分子内13C同位素分布

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This paper presents the improvement of analytical methods for intramolecular carbon isotope compositions (δ13C) of pyruvate. Decarboxylated by H2O2, pyruvate yields acetic acid and CO2. Headspace solid phase micro-extractiongas chromatography-pyrolysis-gas chromatography-combustion-isotope ratio mass spectrometry (HS-SPME-GCPy- GC-C-IRMS) was used to measure the intramolecular δ13C values of acetic acid. δ13C value of CO2 can be later calculated using mass balance equation. The method’s consistency was confirmed by comparison of the δ13C value of CO2 from calculation to its direct measurement. Results of this study confirmed the method improvement because pyruvate 13C intramolecular distribution patterns were obtained. Two intramolecular 13C distribution patterns for commercial chemical reagents were found using this developed method. Intramolecular 13C distribution patterns for pyruvate were found for application in dietary supplements. Its origin was inferred. The method presented herein is expected to be a useful tool for categorization of pyruvate into different intramolecular 13C distribution patterns, which might indicate different production processes or raw materials.
机译：本文提出了丙酮酸分子内碳同位素组成（δ13C）分析方法的改进。通过H2O2脱羧，丙酮酸生成乙酸和CO2。顶空固相微萃取气相色谱-热解气相色谱-燃烧-同位素比质谱（HS-SPME-GCPy-GC-C-IRMS）用于测量乙酸的分子内δ13C值。稍后可以使用质量平衡方程式计算CO2的δ13C值。通过比较从计算到直接测量的CO2的δ13C值，证实了该方法的一致性。这项研究的结果证实了方法的改进，因为获得了丙酮酸13C分子内分布图。使用这种开发的方法发现了两种用于商业化学试剂的分子内13C分布模式。丙酮酸的分子内13C分布模式已发现可用于膳食补充剂。推测其起源。预期本文提出的方法将是用于将丙酮酸分类为不同的分子内13C分布模式的有用工具，其可能指示不同的生产过程或原料。

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