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首页> 外文期刊>Journal of Analytical Science and Technology >A LC–MS/MS method with column coupling technique for simultaneous estimation of lamivudine, zidovudine, and nevirapine in human plasma
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A LC–MS/MS method with column coupling technique for simultaneous estimation of lamivudine, zidovudine, and nevirapine in human plasma

机译：LC-MS / MS色谱柱耦合技术同时估算人血浆中的拉米夫定，齐多夫定和奈韦拉平

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摘要

A new LC–MS/MS method for the simultaneous determination of lamivudine, zidovudine, and nevirapine in human plasma is developed using column-coupling technique. Labeled compound of respective analyte was used as an internal standard. After extraction from 100?μL plasma by solid phase extraction method, analytes were separated on a C18 column coupled with a cation exchange column. Total run time was 4.5?min. A tandem mass spectrometric detection was conducted using multiple reaction monitoring under positive ionization mode with an electrospray ionization interface. The method was validated as per the FDA guidelines over the concentration range of 9.47–1466.67?ng/mL for lamivudine, 10.32–1600.00?ng/mL for zidovudine, and 15.05–2426.67?ng/mL for nevirapine. Precision was in the range 0.86–5.77 (intraday) and 1.92–8.19 (interday) while accuracy was 93.25–104.36?% (intraday) and 96.83–103.28?% (interday). Stabilities of stock in aqueous solutions and in plasma were also determined. The method can be applied to the pharmacokinetic study of a combination treatment.
机译：使用柱耦合技术开发了一种同时测定人血浆中拉米夫定，齐多夫定和奈韦拉平的新LC-MS / MS方法。各个分析物的标记化合物用作内标。通过固相萃取法从100μL血浆中萃取后，在与阳离子交换柱相连的C18柱上分离分析物。总运行时间为4.5分钟。在带有电喷雾电离界面的正电离模式下，使用多反应监测进行串联质谱检测。拉米夫定的浓度范围为9.47-1466.67ng / mL，齐多夫定的浓度范围为10.32-1600.00ng / mL，奈韦拉平的浓度范围为15.05-2426.67ng / mL，该方法已根据FDA指南进行了验证。精度在0.86-5.77（日间）和1.92-8.19（日间）范围内，而精度在93.25-104.36％（日间）和96.83-103.28％（日间）内。还确定了水溶液和血浆中储备液的稳定性。该方法可以应用于联合治疗的药代动力学研究。

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