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首页> 外文期刊>Journal of chromatography, B. Analytical technologies in the biomedical and life sciences >High throughput LC-MS/MS method for simultaneous determination of zidovudine, lamivudine and nevirapine in human plasma
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High throughput LC-MS/MS method for simultaneous determination of zidovudine, lamivudine and nevirapine in human plasma

机译：高通量LC-MS / MS方法同时测定人血浆中的齐多夫定，拉米夫定和奈韦拉平

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摘要

A selective and sensitive high performance liquid chromatography-tandem mass spectrometry method has been developed and validated for simultaneous determination of zidovudine (ZDV), lamivudine (3TC) and nevirapine (NVP) in human plasma. After Solid phase extraction (SPE), analytes and ISTDs were run on Peerless Basic C18 column with an injection volume of 3. μL and run time of 3.0. min. An isocratic mobile phase of 0.1% formic acid in water:methanol (15:85, v/v) was used with positive mass spectrometric detection. The method was validated over a concentration range of 5-1500. ng/mL for ZDV and 3TC and over the concentration range of 10-3000. ng/mL for NVP. The intraday and interday precision and accuracy across four validation runs were ranged from 1.6 to 10.1% and 93.8 to 110.8% respectively.
机译：已开发出一种选择性灵敏的高效液相色谱-串联质谱方法，并已验证了该方法可同时测定人血浆中的齐多夫定（ZDV），拉米夫定（3TC）和奈韦拉平（NVP）。固相萃取（SPE）后，分析物和ISTD在Peerless Basic C18色谱柱上进样，进样体积为3.μL，运行时间为3.0。分钟在水：甲醇（15：85，v / v）中使用0.1％甲酸的等度流动相进行正质谱检测。该方法在5-1500的浓度范围内得到验证。 ZDV和3TC的ng / mL，浓度范围为10-3000。 NVP为ng / mL。四个验证运行的日内和日间准确性和准确性分别为1.6％至10.1％和93.8％至110.8％。

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