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首页> 外文期刊>Journal of Animal Science and Biotechnology >Surplus dietary isoleucine intake enhanced monounsaturated fatty acid synthesis and fat accumulation in skeletal muscle of finishing pigs
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Surplus dietary isoleucine intake enhanced monounsaturated fatty acid synthesis and fat accumulation in skeletal muscle of finishing pigs

机译：过量饮食中异亮氨酸的摄入增强了肥育猪骨骼肌中单不饱和脂肪酸的合成和脂肪的积累

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Isoleucine (Ile) has been implicated in the regulation of energy homeostasis and adipogenesis. However, the impact of surplus dietary Ile intake on muscle lipogenesis remains unknown. The present study aimed to investigate the impact of dietary supplementation of extra-Ile on lipogenesis, fatty acid profile and lipid accumulation in skeletal muscle in finishing pigs. Forty-eight barrows with initial body weight of 77.0?±?0.1?kg were allotted to one of two groups and fed diets containing 0.39%, 0.53% standardized ileal digestible (SID) Ile with six replicates per treatment and four pigs per replicate for 30?d. Dietary Ile intake significantly improved the intramuscular fat (IMF) content and monounsaturated fatty acid (MUFA) concentration in the skeletal muscle (P 0.05). Moreover, the phosphorylation of adenosine monophosphate activated protein kinase α (AMPKα) and acetyl coenzyme A carboxylase (ACC) proteins that monitor lipid metabolism were decreased in skeletal muscle of pigs offered extra-Ile diet (P??0.05). The mRNA expression of adipose-specific genes adipocyte determination and differentiation factor 1 (ADD1), fatty acid synthase (FAS), and stearoyl-CoA desaturase (SCD) were upregulated and the activity of SCD was increased as well (P??0.05). Surplus dietary Ile intake could increase IMF accumulation and MUFA synthesis in skeletal muscle through depressing the phosphorylation of AMPKα-ACC and stimulating the expression and activity of SCD, and increasing the capability of lipogenesis in skeletal muscle.
机译：异亮氨酸（Ile）已参与能量稳态和脂肪形成的调节。但是，饮食中过量摄入Ile对肌肉脂肪生成的影响仍然未知。本研究旨在调查日粮中添加额外的Ile对肥育猪骨骼肌中脂肪生成，脂肪酸谱和脂质蓄积的影响。将初始体重为77.0？±？0.1？kg的四十八只公猪分配给两组之一，并饲喂含有0.39％，0.53％标准化回肠易消化（SID）Ile的日粮，每次处理重复六次，每次重复四头猪30？d饮食中摄入Ile可以显着改善骨骼肌中的肌内脂肪（IMF）含量和单不饱和脂肪酸（MUFA）浓度（P 0.05）。此外，在提供高脂饮食的猪的骨骼肌中，监测脂质代谢的单磷酸腺苷活化蛋白激酶α（AMPKα）和乙酰辅酶A羧化酶（ACC）蛋白的磷酸化降低（P <0.05）。脂肪特异性基因脂肪细胞确定和分化因子1（ADD1），脂肪酸合酶（FAS）和硬脂酰辅酶A去饱和酶（SCD）的mRNA表达上调，并且SCD的活性也增加（P 0.05） ）。饮食中过量摄入Ile可以通过抑制AMPKα-ACC的磷酸化并刺激SCD的表达和活性，以及​​增加骨骼肌的脂肪生成能力，从而增加骨骼肌中IMF的积累和MUFA的合成。

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