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首页> 外文期刊>Journal of Allergy >Inhalation of Ortho-Phthalaldehyde Vapor Causes Respiratory Sensitization in Mice
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Inhalation of Ortho-Phthalaldehyde Vapor Causes Respiratory Sensitization in Mice

机译：吸入邻苯二甲醛蒸气会导致小鼠呼吸致敏。

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Ortho-Phthalaldehyde (OPA) has been approved for high-level sterilization of heat-sensitive medical instruments and is increasingly being used as a replacement in the healthcare industry for glutaraldehyde, a known sensitizer. Numerous case reports have been published indicating workers and patients experiencing respiratory problems, anaphylaxis, skin reactivity, and systemic antibody production. Our laboratory previously demonstrated that OPA is a dermal sensitizer in mice. The goal of the present study was to determine if OPA is a respiratory sensitizer following inhalation exposure. Mice were exposed to OPA vapor and airway and lymph nodes were examined for cytokine gene expression and alterations in lymphocyte populations. Inhalation of OPA for 3 days resulted in a concentration-dependent increase in lymphocyte proliferation, mainly B lymphocytes, in the draining lymph nodes. A secondary challenge of mice with OPA resulted in a dramatic increase in the population of B lymphocytes expressing IgE. Expression of Th2 (IL-4, IL-5, and IL-13) and anti/proinflammatory (IL-10, TNFα, and IL-1β) cytokine genes was upregulated in the lymph nodes and the nasal mucosa. Mice exposed to the higher concentrations of OPA-produced OPA-specific IgG1 antibodies indicating systemic sensitization. These findings provide evidence that OPA has the potential to cause respiratory sensitization in mice.
机译：邻苯二甲醛（OPA）已被批准用于对热敏医疗器械进行高水平灭菌，并且在医疗保健行业中正越来越多地使用邻苯二甲醛替代戊二醛（一种已知的敏化剂）。已经发表了许多病例报告，表明工人和患者遇到呼吸道疾病，过敏反应，皮肤反应性和全身性抗体产生。我们的实验室先前证明OPA是小鼠的皮肤致敏剂。本研究的目的是确定吸入暴露后OPA是否为呼吸道致敏剂。将小鼠暴露于OPA蒸气和气道，并检查淋巴结中细胞因子基因的表达和淋巴细胞群体中的改变。吸入OPA 3天导致引流淋巴结中淋巴细胞增殖（主要是B淋巴细胞）浓度依赖性增加。 OPA小鼠的继发性攻击导致表达IgE的B淋巴细胞数量急剧增加。在淋巴结和鼻粘膜中，Th2（IL-4，IL-5和IL-13）和抗/促炎性细胞因子（IL-10，TNFα和IL-1β）的表达上调。暴露于较高浓度的OPA产生的OPA特异性IgG1抗体的小鼠表现出全身致敏性。这些发现提供了证据表明OPA有可能在小鼠中引起呼吸道致敏。

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