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首页> 外文期刊>Journal of Applied Life Sciences International >A Combination of Curcumin from Turmeric and Alpha-linolenic Acid Shows Antagonism with MCF-7 Breast Cancer Cells in Phenol-red Free Medium
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A Combination of Curcumin from Turmeric and Alpha-linolenic Acid Shows Antagonism with MCF-7 Breast Cancer Cells in Phenol-red Free Medium

机译：姜黄素和姜黄素与α-亚麻酸的组合与酚红免费培养基中的MCF-7乳腺癌细胞显示拮抗作用。

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Aims: To determine the total phenols content and antioxidant capacity for turmeric and curcumin, and to assess the effect of alpha-Linolenic acid (ALA) combinations treatments on MCF-7 breast cancer cell viability and intracellular reactive oxygen species (ROS). Study Design: In-vitro study. Place and Duration of Study: School of Biomedical Sciences, Ulster University, Coleraine (UK) September 2015 to September 2016. Methods: Curcumin was characterized for total phenols content (TPC) and antioxidant capacity (AOC) using Folin-Denis and ABTS (2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid diammonium salt) assays. MCF-7 cells were grown in DMEM phenol-red free medium (+ 10% charcoal stripped foetal bovine serum) and treated with curcumin, ALA or their combinations. Cytotoxicity was assessed using the sulforhodamine-B assay. Intracellular ROS was monitored using 2,7-dichlorodihydroflourescein diacetate assay. Results: Curcumin showed 42-50 folds higher TPC and AOC compared to turmeric. Both curcumin and ALA (0-500 μM) inhibited MCF-7 cells with the 50% effective dose (EC50) equal to 32 μM (curcumin) or 117 μM (ALA). Combination of curcumin and ALA led to EC50 values of 221 μM (curcumin) and 304 μM (ALA). Isobologram analysis and values for Combination index (CI; CI>1.0) are consistent with ALA and curcumin antagonism. Changes of intracellular ROS were 20-fold higher with ALA treatment of MCF-7 compared with curcumin. Conclusions: ALA and curcumin were each cytotoxic towards MCF-7 breast cancer cells but their combination decreases the effectiveness of each agent due to antagonistic interactions. Both ALA and curcumin produce rises in intracellular ROS for MCF-7 cells. The wider implications of such findings is that though dietary antioxidants could be beneficial on their own, antagonistic interaction with ALA, n-3 fatty acids and other ROS generating conventional anti-cancer drug could be of concern.
机译：目的：确定姜黄和姜黄素的总酚含量和抗氧化能力，并评估α-亚麻酸（ALA）组合治疗对MCF-7乳腺癌细胞活力和细胞内活性氧（ROS）的影响。研究设计：体外研究。研究的地点和时间：2015年9月至2016年9月，英国乌尔斯特大学生物医学学院，英国。方法：姜黄素通过Folin-Denis和ABTS表征总酚含量（TPC）和抗氧化能力（AOC）（2 ，2'-叠氮双（3-乙基苯并噻唑啉-6-磺酸二铵盐）测定法，将MCF-7细胞在无苯酚红的DMEM培养基中（+ 10％炭黑去除的胎牛血清）培养，并用姜黄素，ALA处理结果：姜黄素显示TPC和AOC较姜黄高42至50倍，姜黄素和ALA（0- 500μM抑制MCF-7细胞的50％有效剂量（EC50）等于32μM（姜黄素）或117μM（ALA）。姜黄素和ALA的组合导致EC50值为221μM（姜黄素）和304μM（等效线分析法和组合指数值（CI; CI> 1.0） ）与ALA和姜黄素的拮抗作用一致。与姜黄素相比，用ALA处理MCF-7，细胞内ROS的变化高20倍。结论：ALA和姜黄素均对MCF-7乳腺癌细胞具有细胞毒性，但由于拮抗作用，它们的组合降低了每种药物的有效性。 ALA和姜黄素都使MCF-7细胞的细胞内ROS升高。这些发现的更广泛的含义是，尽管饮食中的抗氧化剂本身可能是有益的，但与ALA，n-3脂肪酸和其他产生常规抗癌药的ROS的拮抗作用可能令人担忧。

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