...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Applied Life Sciences International >Effect of Methotrexate and Tea Polyphenols on the Viability and Oxidative Stress in MDA-MB-231 Breast Cancer Cells
【24h】

Effect of Methotrexate and Tea Polyphenols on the Viability and Oxidative Stress in MDA-MB-231 Breast Cancer Cells

机译：甲氨蝶呤和茶多酚对MDA-MB-231乳腺癌细胞活力和氧化应激的影响

获取原文
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Aim: To determine the effect of tea polyphenols and methotrexate on viability and reactive oxygen species (ROS) in a naturally resistant breast cancer cell line MDA-MB-231. Methodology: MDA-MB-231 cells were selected as a model for methotrexate resistant breast cancer. Drug tests were performed over 72 hours at concentrations 0-100 μM. Pre-treatments were with quercetin (QE) or epigallocatechin gallate (EGCG) for 5 hours followed by methotrexate. Cytotoxicity was measured using the MTT assay or resazurin fluorescence assay. ROS was determined using the 2’, 7’-dichlorofluorescein diacetate assay. Intracellular GSH was measured using the monochlorobimane assay. Results: Methotrexate was cytotoxic to MDA-MB-231 cells with IC50 of 35±4 μM. The IC50 value was 68±9.4 μM with QE and 83±16 μM for EGCG. The pre-treatment with QE and EGCG lowered the IC50 for methotrexate by 28% ( P =0.009) and 16% ( P =0.2027). Intracellular ROS concentrations increased after treatment with methotrexate, QE or EGCG singly and ROS decreased with combination treatment compared with the response for methotrexate only. There were no significant changes in intracellular GSH. Conclusion: Pretreatment with tea polyphenols partially sensitized breast cancer cells towards methotrexate and decreases intracellular ROS. More research is needed to optimize the sensitizing effect of tea phenols on the breast cancer cell response to methotrexate.
机译：目的：确定茶多酚和甲氨蝶呤对天然抗性乳腺癌细胞系MDA-MB-231的活力和活性氧（ROS）的影响。方法：选择MDA-MB-231细胞作为耐甲氨蝶呤的乳腺癌模型。药物测试是在72小时内以0-100μM的浓度进行的。预处理是用槲皮素（QE）或表没食子儿茶素没食子酸酯（EGCG）进行5小时，然后进行甲氨蝶呤治疗。使用MTT测定法或刃天青荧光测定法测定细胞毒性。使用2'，7'-二氯荧光素二乙酸盐测定法测定ROS。使用一氯二苯胺测定法测量细胞内GSH。结果：甲氨蝶呤对MDA-MB-231细胞具有细胞毒性，IC 50 为35±4μM。 QE的IC 50 值为68±9.4μM，EGCG的IC 50 值为83±16μM。 QE和EGCG的预处理将甲氨蝶呤的IC 50 降低了28％（P = 0.009）和16％（P = 0.2027）。单用甲氨蝶呤，QE或EGCG处理后，与仅对甲氨蝶呤的反应相比，单用QE或EGCG处理后细胞内ROS浓度升高，而ROS降低。细胞内GSH没有明显变化。结论：茶多酚预处理可使乳腺癌细胞对甲氨蝶呤敏感，并降低细胞内ROS。需要更多的研究来优化茶酚对乳腺癌细胞对甲氨蝶呤反应的敏化作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号