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首页> 外文期刊>Journal of Biological Researches >Biofilm formation and detection of A/D genes in MRSA (Methicillin-Resistant Staphylococcus aureus) and MSSA (Methicillin-Sensitive Staphylococcus aureus)
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Biofilm formation and detection of A/D genes in MRSA (Methicillin-Resistant Staphylococcus aureus) and MSSA (Methicillin-Sensitive Staphylococcus aureus)

机译：MRSA（耐甲氧西林金黄色葡萄球菌）和MSSA（耐甲氧西林金黄色葡萄球菌）的生物膜形成和A / D基因检测

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MRSA (Methicillin-Resistant Staphylococcus aureus) arises due to excessive use of methicillin antibiotics and complications from all medical practices that have been carried out lately. The prevalence of MRSA tends to increase and its ability to form biofilms so that it has the potential to significantly increase mortality and morbidity. Biofilms are considered one of the microbial virulence factors. Biofilm formation in S. aureus is regulated by the expression of PIA which mediates attachment between cells to cells and is a product of the ABDC ica gene. This study aims to determine the ability of biofilm formation as one of the virulence factors in MRSA compared to MSSA (Methicillin-Sensitive Staphylococcus aureus) . its relationship to the presence of A and D ica genes as biofilm coding genes. Biofilm formation assay on MRSA and MSSA using the Microtiter Plate (MtP) method and the A/D gene detection assay performed by PCR method. The results showed that the incubation end value of Optical Density (OD) for MSSA1 (0.75), MSSA2 (0.46), MRSA1 (0.53) isolates according to the formula were interpreted as moderate biofilm producer. The conclusions of this study were all of the test isolates (MSSA1, MSSA2, and MRSA1) were able to form a biofilm. Distribution of ica A and ica D genes in MSSA 1 and MSSA 2 was varied while MRSA1 had the ica A and ica D genes.
机译：MRSA（耐甲氧西林金黄色葡萄球菌）的产生是由于过量使用甲氧西林抗生素和最近进行的所有医疗操作引起的并发症。 MRSA的患病率趋于增加，其形成生物膜的能力趋于增加，因此有可能显着增加死亡率和发病率。生物膜被认为是微生物毒力因子之一。金黄色葡萄球菌中生物膜的形成受PIA表达的调节，PIA介导细胞与细胞之间的附着，是ABDC ica基因的产物。这项研究旨在确定与MSSA（对甲氧西林敏感的金黄色葡萄球菌）相比，生物膜形成作为MRSA中毒力因子之一的能力。它与存在A和D ica基因作为生物膜编码基因的关系。使用微量滴定板（MtP）方法在MRSA和MSSA上进行生物膜形成测定，并通过PCR方法进行A / D基因检测测定。结果表明，根据该公式，MSSA1（0.75），MSSA2（0.46），MRSA1（0.53）菌株的光密度（OD）的孵育终值被认为是中等的生物膜生产者。这项研究的结论是所有测试分离株（MSSA1，MSSA2和MRSA1）都能够形成生物膜。 ica A和ica D基因在MSSA 1和MSSA 2中的分布有所不同，而MRSA1具有ica A和ica D基因。

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