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首页> 外文期刊>The Journal of automatic chemistry >Analysis of the High-Performance Liquid Chromatography Fingerprints and Quantitative Analysis of Multicomponents by Single Marker of Products of Fermented Cordyceps sinensis
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Analysis of the High-Performance Liquid Chromatography Fingerprints and Quantitative Analysis of Multicomponents by Single Marker of Products of Fermented Cordyceps sinensis

机译：冬虫夏草产品的高效液相色谱指纹图谱和多组分定量分析

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Fermented Cordyceps sinensis, the succedaneum of Cordyceps sinensis which is extracted and separated from Cordyceps sinensis by artificial fermentation, is commonly used in eastern Asia in clinical treatments due to its health benefit. In this paper, a new strategy for differentiating and comprehensively evaluating the quality of products of fermented Cordyceps sinensis has been established, based on high-performance liquid chromatography (HPLC) fingerprint analysis combined with similar analysis (SA), hierarchical cluster analysis (HCA), and the quantitative analysis of multicomponents by single marker (QAMS). Ten common peaks were collected and analysed using SA, HCA, and QAMS. These methods indicated that 30 fermented Cordyceps sinensis samples could be categorized into two groups by HCA. Five peaks were identified as uracil, uridine, adenine, guanosine, and adenosine, and according to the results from the diode array detector, which can be used to confirm peak purity, the purities of these compounds were greater than 990. Adenosine was chosen as the internal reference substance. The relative correction factors (RCF) between adenosine and the other four nucleosides were calculated and investigated using the QAMS method. Meanwhile, the accuracy of the QAMS method was confirmed by comparing the results of that method with those of an external standard method with cosines of the angles between the groups. No significant difference between the two methods was observed. In conclusion, the method established herein was efficient, successful in identifying the products of fermented Cordyceps sinensis, and scientifically valid to be applicable in the systematic quality control of fermented Cordyceps sinensis products.
机译：冬虫夏草的发酵产物，是通过人工发酵从冬虫夏草中提取并分离出来的冬虫夏草，由于其有益于健康，因此在东亚被广泛用于临床治疗。本文基于高效液相色谱（HPLC）指纹图谱分析，相似分析（SA），层次聚类分析（HCA），建立了区分和综合评价发酵冬虫夏草产品质量的新策略。 ，以及通过单一标记（QAMS）对多组分进行定量分析。使用SA，HCA和QAMS收集并分析了十个常见峰。这些方法表明，通过HCA可以将30个发酵的冬虫夏草样品分为两组。鉴定出了五个峰，分别为尿嘧啶，尿苷，腺嘌呤，鸟苷和腺苷，根据二极管阵列检测器的结果可用于确认峰纯度，这些化合物的纯度均大于990。内部参考物质。使用QAMS方法计算并研究了腺苷和其他四个核苷之间的相对校正因子（RCF）。同时，通过比较组间角度的余弦值，将该方法的结果与外标方法的结果进行比较，从而确认了QAMS方法的准确性。两种方法之间未观察到显着差异。综上所述，本文建立的方法有效，成功地鉴定了冬虫夏草产品，在科学上可用于冬虫夏草产品的系统质量控制。

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