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首页> 外文期刊>Journal of Behavioral and Brain Science >The Neuroprotective Effect of Picroside II and Its Best Therapeutic Dose and Time Window in Cerebral Ischemic Injury in Rats
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The Neuroprotective Effect of Picroside II and Its Best Therapeutic Dose and Time Window in Cerebral Ischemic Injury in Rats

机译：Picroside II对大鼠脑缺血损伤的神经保护作用及其最佳治疗剂量和时间窗

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Objective: To study the neuroprotective effect of picrosede II and explore the best therapeutic dose and time window according to orthogonal design in cerebral ischemic injury in rats. Methods: The forebrain ischemia rat models were established by bilateral common carotid artery occlusion (BCCAO) method. The successful models were randomly grouped according to orthogonal experimental design and treated by injecting picroside II intraperitoneally at different ischemic time with different doses. The contents of neuron-specific enolase (NSE), neuroglial marker protein S100B and myelin basic protein (MBP) in serum and brain tissue were determined by enzyme linked immunosorbent assay (ELISA) to evaluate the therapeutic effect of picroside II in cerebral ischemic injury. Results: The best therapeutic time window and dose of picroside II in cerebral ischemic injury may be 1) ischemia 1.5 h with 20 mg/kg and ischemia 1.5 h with 10 mg/kg body weight according to the content of NSE in serum and brain tissue respectively, 2) ischemia 1.5 h with 20 mg/kg according to the content of S100B in both serum and brain tissue, and 3) ischemia 1.5 h with 20 mg/kg and ischemia 1.5 h with 10 mg/kg according to the content of MBP in serum and brain tissue respectively. Conclusion: Based on the principle of the minimization of therapeutic drug dose and maximization of therapeutic time window, the optimal composition of the therapeutic dose and time window of picroside II in treating cerebral ischemic injury should be achieved by injecting picroside II intraperitoneally with 10-20 mg/kg body weight at ischemia 1.5 h in cerebral ischemic injury in rats.
机译：目的：根据正交试验研究吡卡酯II对大鼠脑缺血的神经保护作用，探讨最佳治疗剂量和时间窗。方法：采用双侧颈总动脉闭塞（BCCAO）方法建立前脑缺血大鼠模型。根据正交实验设计将成功的模型随机分组，并在不同的缺血时间，不同剂量腹膜内注射苦味子苷II进行治疗。用酶联免疫吸附法（ELISA）测定血清和脑组织中神经元特异性烯醇化酶（NSE），神经胶质标记蛋白S100B和髓鞘碱性蛋白（MBP）的含量，以评估苦瓜苷II在脑缺血性损伤中的治疗作用。结果：根据不同血清和脑组织中NSE的含量，在大鼠缺血性脑损伤中，苦味苦苷II的最佳治疗时间窗和剂量可能是1）缺血1.5 h 20 mg / kg体重和1.5 h 10 mg / kg体重。根据血清和脑组织中S100B的含量分别为2）缺血1.5 h和20 mg / kg，以及3）根据10mg / kg的含量，缺血20 h / min缺血1.5 h和20 mg / kg MBP分别在血清和脑组织中。结论：基于最小化药物剂量和最大化治疗时间窗的原则，应通过腹膜内注射10-20倍的苦味子苷II来达到最佳剂量的苦味子苷II治疗脑缺血性损伤。大鼠脑缺血损伤1.5 h时的mg / kg体重。

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