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首页> 外文期刊>Journal of Biological Control >Molecular Characterization of?Bacillus isolates?with Insecticidal Activity against Greater Wax Moth,?Galleria mellonella?L. (Pyralidae: Lepidoptera)
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Molecular Characterization of?Bacillus isolates?with Insecticidal Activity against Greater Wax Moth,?Galleria mellonella?L. (Pyralidae: Lepidoptera)

机译：具有杀虫活性的大芽孢杆菌，Galleria mellonella？L的芽孢杆菌分离株的分子特征。 （P科：鳞翅目）

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Six local Bacillus strains isolated from soils from different parts of Karnataka were characterized by their high toxicity against third instar larvae of greater wax moth (Galleria mellonella?L.). The PCR amplification analysis using B. thuringiensis specific primers revealed that only four isolates are Bt isolates. The PCR amplification analysis for cry genes indicated the presence of amplified fragments characteristic of three different cry genes cry1Aa, cry2Aa and cry11Aa. Results showed the presence of cry11Aa and cry1Aa in isolate N12 with expected sizes of 816bp and 891-1089bp, respectively. Bt isolate; A-7 harbored cry1Aa, cry2Aa and cry11Aa with product size 891-1308, 235-696 and 636bp, respectively. While the isolates M-2 and A3 contained only one gene cry2Aa with a product size 696-735bp. The two isolates; N-12 and A-7 were harbored multiple cry genes. The cry2Aa genes were the most frequently found among the three local Bt isolates. The profiles of cry genes indicated that all isolates were active against lepidopteran insects, and therefore these strains could be potential candidates in the search for biocontrol agents in management of greater wax moth,?G. mellonella?with specific lepidopteran activity.
机译：从卡纳塔克邦不同地区的土壤中分离出的六株本地芽孢杆菌菌株，其特点是对较大的蜡蛾（Galleria mellonella？L。）第三龄幼虫具有高毒性。使用苏云金芽孢杆菌特异性引物的PCR扩增分析表明，只有四个分离株是Bt分离株。对cry基因的PCR扩增分析表明存在三种不同的cry基因cry1Aa，cry2Aa和cry11Aa特征性扩增片段。结果显示，分离株N12中存在cry11Aa和cry1Aa，预期大小分别为816bp和891-1089bp。 Bt隔离; A-7包含cry1Aa，cry2Aa和cry11Aa，产品大小分别为891-1308、235-696和636bp。而分离物M-2和A3仅包含一个基因cry2Aa，产物大小为696-735bp。两个分离株; N-12和A-7带有多个cry基因。 cry3Aa基因是在三个本地Bt分离株中最常见的基因。 cry基因的特征表明所有分离株均对鳞翅目昆虫具有活性，因此这些菌株可能是寻找更大蜡蛾ΔG的生物防治剂的潜在候选者。具鳞翅目活动的mellonella？

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