...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of biosciences >Differential cytotoxic effects of Annona squamosa seed extracts on human tumour cell lines: Role of reactive oxygen species and glutathione
【24h】

Differential cytotoxic effects of Annona squamosa seed extracts on human tumour cell lines: Role of reactive oxygen species and glutathione

机译：番荔枝核种子提取物对人肿瘤细胞系的不同细胞毒性作用：活性氧和谷胱甘肽的作用

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Annonaceous acetogenins are a new class of compounds that have been reported to have potent pesticidal, parasiticidal, anti-microbial, cell growth inhibitory activities. In this study, organic and aqueous extracts from the defatted seeds of Annona squamosa (custard apple) were tested on different human tumour cell lines for antitumoural activity. While organic and aqueous extracts induced apoptosis in MCF-7 and K-562 cells, they failed to do so in COLO-205 cells. Treatment of MCF-7 and K-562 cells with organic and aqueous extracts resulted in nuclear condensation, DNA fragmentation, induction of reactive oxygen species (ROS) generation and reduced intracellular glutathione levels. In addition downregulation of Bcl-2 and PS externalization by Annexin-V staining suggested induction of apoptosis in MCF-7 and K-562 cells by both the extracts through oxidative stress. On the contrary, COLO-205 cells showed only PS externalization but no change in ROS and glutathione levels. These observations suggest that the induction of apoptosis by A. squamosa extracts can be selective for certain types of cancerous cells.
机译：丙酮酸非生成素是一类新型化合物，据报道具有有效的杀虫，杀虫，抗微生物，抑制细胞生长的活性。在这项研究中，在不同的人类肿瘤细胞系上测试了脱脂的番荔枝番荔枝种子的有机提取物和水提取物的抗肿瘤活性。尽管有机提取物和水提取物在MCF-7和K-562细胞中诱导凋亡，但在COLO-205细胞中却没有。用有机提取物和含水提取物处理MCF-7和K-562细胞会导致核浓缩，DNA断裂，诱导活性氧（ROS）生成并降低细胞内谷胱甘肽水平。此外，膜联蛋白-V染色对Bcl-2和PS外在化的下调表明，两种提取物均通过氧化应激诱导MCF-7和K-562细胞凋亡。相反，COLO-205细胞仅显示PS外在化，而ROS和谷胱甘肽水平没有变化。这些观察结果表明，鳞球曲霉提取物诱导的细胞凋亡对某些类型的癌细胞具有选择性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号