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首页> 外文期刊>Journal of Botany >Nuclei ofTaxus baccata: Flavanols Linked to Chromatin Remodeling Factors
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Nuclei ofTaxus baccata: Flavanols Linked to Chromatin Remodeling Factors

机译：芽孢杆菌核：黄烷醇与染色质重塑因子相关

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Microscopic studies of young needles and shoot tips fromTaxus baccatashowed that flavanols are localized in the nuclei. This observation is based on the histochemical staining of flavanols with the DMACA reagent. The colour that is obtained with this reagent varies from pale to deep blue, depending on the amount of flavanols. This study is focused on nondifferentiated cell lineages and on differentiating cells. The key point to note is that all nuclei of a cell lineage showed a uniform DMACA staining pattern based on the amount and structural appearence of nuclear flavanols. This points to transcriptional and epigenetic programming. However, comparing various cell lineages from different shoot tips and needles revealed a lineage-specific expression of nuclear flavanols. This result implied that both positional and developmental signals from neighbouring cells were involved in the nuclear flavanol binding of lineages. The cells of a developmentally advanced lineage loose their intimate contact and, then, they separate from each other to undergo an autonomous, individual sequence of differentiation. This in turn was accompanied by differences in the nuclear flavanol patterns of the single cells. Investigating different mitotic stages revealed a wide spectrum in flavanol staining intensities of the chromosomes. These observations should be linked to UV-VIS spectroscopical kinetic results indicating that nuclear flavanols bound to histones are involved in epigenetically regulated modification of chromatin. The kinetic studies show that catechin is relatively rapidly degraded by oxygen in the presence ofMg2+-ions. However, this degradation reaction is strongly inhibited when histone proteins were added. This behaviour is a clear indication that coregulatory interactions exist between catechin and histones.
机译：对来自Taxus baccata的幼针和芽尖的显微研究表明，黄烷醇位于核内。该观察结果是基于用DMACA试剂对黄烷醇进行的组织化学染色。用这种试剂获得的颜色从浅到深蓝色不等，具体取决于黄烷醇的含量。这项研究的重点是未分化细胞谱系和分化细胞。要注意的关键点是，根据核黄烷醇的含量和结构外观，细胞谱系的所有核均显示出均匀的DMACA染色模式。这指向转录和表观遗传编程。然而，比较来自不同芽尖和针的各种细胞谱系揭示了核黄烷醇的谱系特异性表达。该结果暗示来自邻近细胞的位置和发育信号均参与谱系的核黄烷醇结合。发育先进的谱系的细胞失去了紧密的接触，然后彼此分离，经历了自主的，独立的分化序列。这又伴随着单个细胞核黄烷醇模式的差异。研究不同的有丝分裂阶段揭示了染色体的黄烷醇染色强度范围广。这些观察结果应与UV-VIS光谱动力学结果联系起来，表明与组蛋白结合的核黄烷醇参与了染色质的表观遗传调控。动力学研究表明，在Mg2 +离子存在下，儿茶素被氧气相对迅速地降解。然而，当加入组蛋白时，这种降解反应被强烈抑制。此行为清楚地表明儿茶素和组蛋白之间存在协同调节作用。

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