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首页> 外文期刊>Journal of Botany >Icelandic Birch Polyploids—The Case of a Perfect Fit in Genome Size
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Icelandic Birch Polyploids—The Case of a Perfect Fit in Genome Size

机译：冰岛桦木多倍体—基因组大小完美匹配的案例

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Two birch species coexist in Iceland, dwarf birchBetula nanaand tree birchB. pubescens. Both species are variable morphologically, which has been shown to be due to introgressive hybridization via interspecific hybrids. The aim of this study was to examine if the introgression could be related to genome size. We characterized 42 plants from Bifröst woodland morphologically and cytogenetically. The population consisted of diploidB. nana(38%), tetraploidB. pubescens(55%), and triploid hybrids (7%). Genome size was measured from 12 plants, using Feulgen DNA image densitometry (FDM) on spring leaf buds and flow cytometry (FCM) with dormant winter twigs. The use of winter twigs for FCM is novel. The average 1C-values for diploid, triploid, and tetraploid plants were 448, 666, and 882 Mbp, respectively. Monoploid genome sizes were found to be statistically constant among ploidy levels. This stability is in contrast to the different taxonomic positions of the di- and tetraploids and also contrasts with the frequent occurrence of genome downsizing in polyploids.
机译：矮人桦，桦桦和桦树桦在冰岛共存两种桦树种。 pubescens。两种物种在形态上都是可变的，这已经证明是由于通过种间杂种进行的渐渗杂交。这项研究的目的是检查基因渗入是否与基因组大小有关。我们对Bifröst林地的42种植物进行了形态和细胞遗传学鉴定。该群体由二倍体B组成。娜娜（38％），四倍体B。 （55％）和三倍体杂种（7％）。使用春季叶芽上的Feulgen DNA图像密度测定法（FDM）和带有休眠冬枝的流式细胞术（FCM），测量了12种植物的基因组大小。将冬季树枝用于FCM是新颖的。二倍体，三倍体和四倍体植物的平均1C值分别为448、666和882 Mbp。发现单倍体基因组大小在倍数水平之间在统计学上是恒定的。这种稳定性与二倍体和四倍体的不同生物分类位置相反，也与多倍体中基因组小型化的频繁发生形成对比。

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