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首页> 外文期刊>Journal of Botany >Analysis of Genetic Diversity inAstragalus rhizanthusBenth. ssp.rhizanthusvar.rhizanthus(Fabaceae) Using Molecular Markers from India
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Analysis of Genetic Diversity inAstragalus rhizanthusBenth. ssp.rhizanthusvar.rhizanthus(Fabaceae) Using Molecular Markers from India

机译：黄芪的遗传多样性分析。 ssp.rhizanthusvar.rhizanthus（Fabaceae）使用来自印度的分子标记

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Astragalus rhizanthuswith three infraspecific taxa (i.e.,A. rhizanthusssp.rhizanthusvar.rhizanthus, A.rhizanthusssp.rhizanthusvar.pindreensis,andA. rhizanthusssp.candolleanus) is widely scattered in the Himalaya from Jammu and Kashmir to Uttarakhand provinces in India. Among them,A. rhizanthusssp.rhizanthusvar.rhizanthusexhibits enormous morphological diversity throughout its range of distribution. An assessment of genetic diversity studies was undertaken to understand the level and pattern of diversity, using Inter simple sequence repeats (ISSR), Random amplified polymorphic DNA (RAPD) and Directed amplification of minisatellite DNA (DAMD) profiles. Fifteen ISSR, 18 RAPD and 6 DAMD primers were used to unravel the diversity among 20 genotypes collected from the known localities in the Indian Himalaya. A total of 242 bands from ISSR, 352 from RAPD and 142 from DAMD were obtained with an average of 92.23% polymorphism in the species. Pair-wise genetic similarity for the cumulative data was determined using Jaccard's similarity coefficient which varied from 0.19 to 0.84. A combined UPGMA dendrogram was generated which revealed that different genotypes exhibited their affinity according to their geographical distribution. Tree topology suggests the existence of two distinct groups of the genotypes. Gene diversity(H)and Shannon's information index (I) were estimated and these values were found higher in the genotypes collected from Jammu and Kashmir than Himachal Pradesh.
机译：在印度的喜马拉雅山，查Jam和喀什喀什邦普遍分布着带有三个亚种的类群黄芪（即：A。rhizanthusssp.rhizanthusvar.rhizanthus，A.rhizanthusssp.rhizanthusvar.pindreensis和A. rhizanthusssp.candolleanus）。其中，A。 Rhizanthusssp.rhizanthusvar.rhizanthuse在整个分布范围内表现出巨大的形态多样性。进行了一项遗传多样性研究评估，以了解使用简单序列间重复（ISSR），随机扩增多态性DNA（RAPD）和小卫星DNA定向扩增（DAMD）谱来了解多样性的水平和模式。使用15种ISSR，18种RAPD和6种DAMD引物揭示了从印度喜马拉雅山已知地区收集的20个基因型之间的多样性。该物种共获得242条来自ISSR的条带，352条来自RAPD的条带和142条来自DAMD的条带，平均该物种的多态性为92.23％。累积数据的成对遗传相似性是使用Jaccard相似性系数（介于0.19至0.84之间）确定的。生成了组合的UPGMA树状图，该图表明不同的基因型根据其地理分布表现出亲和力。树的拓扑结构表明存在两个不同的基因型组。估计基因多样性（H）和香农信息指数（I），发现这些值在查Jam和克什米尔采集的基因型中高于喜马al尔邦。

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