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首页> 外文期刊>Journal of Cancer Research and Treatment >The Antioxidant Glycolysis Inhibitor (Citric Acid) Induces a Dose-dependent Caspase-mediated Apoptosis and Necrosis in Glioma Cells
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The Antioxidant Glycolysis Inhibitor (Citric Acid) Induces a Dose-dependent Caspase-mediated Apoptosis and Necrosis in Glioma Cells

机译：抗氧化剂糖酵解抑制剂（柠檬酸）诱导胶质瘤细胞中剂量依赖性胱天蛋白酶介导的细胞凋亡和坏死。

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Background: Glioma tumors are still a big challenge being incurable with current chemotherapy and radiotherapy treatments. Surgical treatment of glioma needs adjuvant effective targeting therapy for better glioma cell treatment. Citrate is a well-known antioxidant organic acid abundant in citrus fruits and is an inhibitor of glycolysis through targeting the glycolytic enzyme phosphofructokinase, one of the key enzymes of glycolysis. Citrate is a natural product that is formed inside mitochondria during Krebs cycle to the extent that Krebs cycle is often referred to as citric acid cycle. It was reported that glioma cells are driven by glycolysis where glioma cells upregulates the expression of glycolysis genes and enzymes. Objectives: This aim is to investigate effect of citrate on glioma cells viability, morphology and moge of glioma-induced cell death. Methodology: In this study, citrate-induced glioma cell death was investigated using MTT assay, western blot analysis and flowcytometric evaluation was done to C6 glioma cells. Results: Citrate induced a potent anti-glioma effect by significantly decreasing viability of C6 glioma cells in a dose-dependent manner. Flow cytometric analysis revealed that at 5 mM, citrate induced a caspase-dependent apoptotic glioma cell death. Higher doses of citrate (9 mM) induced necro-apoptotic glioma cell death. Conclusion: citrate may be a promising therapeutic treatment for glioma and glioblastoma. Citrate-rich fruits are strongly recommended as a nutritional treatment for glioma patients.
机译：背景：神经胶质瘤肿瘤仍是当前化疗和放射治疗无法治愈的巨大挑战。胶质瘤的外科治疗需要辅助的有效靶向治疗，以更好地治疗胶质瘤细胞。柠檬酸盐是在柑橘类水果中丰富的众所周知的抗氧化剂有机酸，并且通过靶向糖酵解酶磷酸果糖激酶（一种糖酵解的关键酶）而成为糖酵解的抑制剂。柠檬酸盐是在克雷布斯循环期间在线粒体内形成的天然产物，其程度通常将克雷布斯循环称为柠檬酸循环。据报道，神经胶质瘤细胞由糖酵解驱动，其中神经胶质瘤细胞上调糖酵解基因和酶的表达。目的：本研究旨在研究柠檬酸盐对神经胶质瘤细胞活力，神经胶质瘤诱导的细胞死亡形态和形态的影响。方法：在这项研究中，使用MTT分析法研究了柠檬酸盐诱导的神经胶质瘤细胞死亡，对C6胶质瘤细胞进行了蛋白质印迹分析和流式细胞术评估。结果：柠檬酸盐通过以剂量依赖性方式显着降低C6胶质瘤细胞的活力，诱导了有效的抗胶质瘤作用。流式细胞仪分析表明，在5 mM时，柠檬酸盐诱导了caspase依赖性凋亡神经胶质瘤细胞死亡。更高剂量的柠檬酸盐（9 mM）诱导坏死性凋亡神经胶质瘤细胞死亡。结论：柠檬酸盐可能是治疗神经胶质瘤和胶质母细胞瘤的一种有前途的治疗方法。强烈建议将富含柠檬酸盐的水果作为神经胶质瘤患者的营养治疗方法。

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