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首页> 外文期刊>Journal of Cancer Research and Therapeutics >Increased expression of epidermal growth factor-like domain-containing protein 7 is predictive of poor prognosis in patients with hepatocellular carcinoma
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Increased expression of epidermal growth factor-like domain-containing protein 7 is predictive of poor prognosis in patients with hepatocellular carcinoma

机译：表皮生长因子样结构域蛋白7的表达增加预示肝细胞癌患者预后不良

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Objective: Epidermal growth factor-like domain-containing protein 7 (EGFL7) is an endothelial cell-derived secreted factor that regulates vascular tube formation. In human cancer, the specificity of expression is lost as EGFL7 has been detected in tumor cells, in addition to endothelial cells. This study evaluated the intricate relationship between hypoxia-inducible factor 1-alpha (HIF-1α) and EGFL7 under both hyperoxia and hypoxia states. Materials and Methods: In the present study, immunohistochemical staining and ELISA were applied to examine the relative level of EGFL7 in 182 cases of hepatocellular carcinoma (HCC) formalin-fixed and paraffin-embedded tissues and 110 cases of HCC serum samples. Quantitative polymerase chain reaction and Western blotting were applied to verify the correlation between serum EGFL7 level and anoxic microenvironment. Immunohistochemical staining was performed to determine the correlation between EGFL7 and HIF1-α. Results: The correlations between EGFL7 expression and patients' age, tumor size, gender, N-stage, history of cirrhosis, M-stage, history of hepatitis C, and history of hepatitis B were statistically insignificant (P = 0.28, 0.34, 0.71, 0.15, 0.8, 0.2, 0.052, and 0.14, respectively). High level of EGFL7 was significantly correlated with overall survival as well as disease-free survival in 182 HCC patients (P = 0.0016 and P P P Conclusions: EGFL7 was found to be a potential predictor for HCC survival and metastasis state; EGFL7 may be a promising biomarker and therapeutic target in human HCC.
机译：目的：表皮生长因子样结构域含有蛋白7（EGFL7）是内皮细胞衍生的分泌因子，可调节血管的形成。在人类癌症中，表达的特异性丧失了，因为除内皮细胞外，还在肿瘤细胞中检测到了EGFL7。这项研究评估了在高氧和低氧状态下低氧诱导因子1-alpha（HIF-1α）和EGFL7之间的复杂关系。材料与方法：本研究采用免疫组织化学染色和ELISA法检测182例肝细胞福尔马林固定和石蜡包埋组织和110例HCC血清样本中EGFL7的相对水平。应用定量聚合酶链反应和蛋白质印迹法验证血清EGFL7水平与缺氧微环境之间的相关性。进行免疫组织化学染色以确定EGFL7和HIF1-α之间的相关性。结果：EGFL7表达与患者年龄，肿瘤大小，性别，N期，肝硬化病史，M期，丙型肝炎病史和乙型肝炎病史之间的相关性无统计学意义（P = 0.28、0.34、0.71） ，分别为0.15、0.8、0.2、0.052和0.14）。高水平的EGFL7与182例HCC患者的总生存率和无病生存率显着相关（P = 0.0016和PPP结论）：发现EGFL7是HCC生存和转移状态的潜在预测指标; EGFL7可能是有希望的生物标志和人类肝癌的治疗靶标。

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