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首页> 外文期刊>Journal of Clinical & Translational Endocrinology >Comprehensive assessment of expression of insulin signaling pathway components in subcutaneous adipose tissue of women with and without polycystic ovary syndrome
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Comprehensive assessment of expression of insulin signaling pathway components in subcutaneous adipose tissue of women with and without polycystic ovary syndrome

机译：综合评估有无多囊卵巢综合征的妇女皮下脂肪组织中胰岛素信号通路成分的表达

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Objective Insulin resistance is a common feature of polycystic ovary syndrome (PCOS). The insulin signaling pathway consists of two major pathways, the metabolic and the mitogenic cascades. The many components of these pathways have not been comprehensively analyzed for differential expression in insulin-responsive tissues in PCOS. The goal of this study was to determine whether the core elements of the insulin signal transduction cascade were differentially expressed in subcutaneous adipose tissue (SAT) between PCOS and controls. Materials/methods Quantitative real-time PCR for 36 insulin signaling pathway genes was performed in subcutaneous adipose tissue from 22 white PCOS and 13 healthy controls. Results Genes in the insulin signaling pathway were not differentially expressed in subcutaneous adipose tissue between PCOS and controls ( P ?>?0.05 for all). Components mainly of the mitogenic pathway were correlated with both androgens and metabolic phenotypes. Expression levels of five genes ( MKNK1 , HRAS , NRAS , KRAS , and GSK3A ) were positively correlated with total testosterone level ( ρ ?>?0, P ?
机译：目的胰岛素抵抗是多囊卵巢综合征（PCOS）的共同特征。胰岛素信号传导途径包括两个主要途径，代谢途径和有丝分裂级联反应。这些途径的许多组成部分尚未针对PCOS中胰岛素反应性组织中的差异表达进行全面分析。这项研究的目的是确定PCOS与对照之间的皮下脂肪组织（SAT）中胰岛素信号转导级联的核心元件是否差异表达。材料/方法在22个白色PCOS和13个健康对照的皮下脂肪组织中进行了36种胰岛素信号通路基因的实时定量PCR。结果胰岛素信号通路中的基因在PCOS和对照组之间的皮下脂肪组织中没有差异表达（所有P≥0.05）。有丝分裂途径的主要成分与雄激素和代谢表型均相关。五个基因（MKNK1，HRAS，NRAS，KRAS和GSK3A）的表达水平与总睾丸激素水平呈正相关（ρ> 0，P <0.05）。发现六个基因（HRAS，MAP2K2，NRAS，MAPK3，GRB2和SHC1）的表达与代谢特征（体重指数，空腹血糖，空腹胰岛素和HOMA-IR）呈负相关（ρ≤<0，P ＜0.05。结论皮下脂肪组织中核心胰岛素信号通路成分的差异表达并不是PCOS发病的主要因素。临床表型与胰岛素信号传导途径的有丝分裂肢体中若干基因表达之间的相关性表明，胰岛素的有丝分裂信号传导可能调节类固醇生成和葡萄糖稳态。强调 ？我们检查了PCOS与对照之间脂肪组织中的基因表达。 ？完整覆盖胰岛素信号传导途径的核心要素。 ？脂肪组织中差异mRNA的表达不是PCOS的主要贡献者。 ？我们发现有丝分裂信号成分和表型之间的关联。

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