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首页> 外文期刊>Journal of Clinical Medicine Research >Evaluation of Immune Responses to Seasonal Influenza Vaccination in Healthy Volunteers in South Apulia, Italy: A Pilot Study
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Evaluation of Immune Responses to Seasonal Influenza Vaccination in Healthy Volunteers in South Apulia, Italy: A Pilot Study

机译：意大利南部普利亚健康志愿者对季节性流感疫苗免疫反应的评估：一项初步研究

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Background: The aim of our pilot study is to investigate different components of the immune response to influenza vaccination in a group of healthy volunteers. We evaluated the cellular immune response (CD4+ T lymphocytes) by flow cytometry. The humoral immune response was assessed by measuring the serum haemagglutination inhibition antibody response.?Methods: Healthy adult donors (n = 18), were vaccinated with a commercially influenza virus vaccine (FLUARIX? GlaxoSmithKline S.p.a. Verona, Italy), peripheral blood was drawn the same day as influenza virus vaccination and one month later in order to enumerate the antigen-specific CD4+ T lymphocytes. Hemagglutination inhibition assay was performed to enumerate the titer of neutralizing antibodies. Samples of nasal-pharyngeal secretions were taken by swabbing, from ILI (Influenza like Illness) subjects among the studied group, in order to verify influenza infections and eventually identify viruses using Real Time PCR.?Results and Conclusions: Parenteral influenza vaccination results in significant increase in the CD4+ Th cell population after vaccination. The number of pre-vaccination CD4+ T cells was 0.018 [the results are presented as number of percent fluorescent cells per 10 000 lymphocytes (fixed cells)], while there was a significantly higher number of CD4+ Th cells one month after vaccination (statistical significance was set at the level of α = 0.01). Twenty-two percent of patients demonstrated protective antibody levels to influenza A H1/N1 serotype. None was diagnosed with influenza type A or B.doi:10.4021/jocmr647w
机译：背景：我们的初步研究旨在研究一组健康志愿者对流感疫苗免疫反应的不同组成部分。我们通过流式细胞术评估了细胞免疫应答（CD4 + T淋巴细胞）。方法：通过将健康的成人供体（n = 18）接种商业流感病毒疫苗（FLUARIX？GlaxoSmithKline Spa Verona，Italy），给健康的成人供体（n = 18）接种疫苗。流感疫苗接种的同一天，一个月后，以列举抗原特异性CD4 + T淋巴细胞。进行血凝抑制测定以计算中和抗体的效价。通过擦拭从研究组的ILI（流感样疾病）受试者中鼻咽分泌物样本，以验证流感感染并最终使用实时PCR鉴定病毒。结果与结论：肠胃外疫苗接种的结果显着疫苗接种后CD4 + Th细胞数量增加。疫苗接种前CD4 + T细胞的数量为0.018 [结果表示为每万个淋巴细胞（固定细胞）的荧光细胞百分比]，而疫苗接种后一个月CD4 + Th细胞的数量明显更高（统计学意义设定为α= 0.01）。 22％的患者表现出针对甲型H1 / N1流感血清型的保护性抗体水平。没有人被诊断出患有A型或B型流感。doi：10.4021 / jocmr647w

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