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首页> 外文期刊>Journal of Clinical Immunology and Immunopathology Research >Cytokine levels associated with experimental malaria pathology during Plasmodium berghei ANKA infection in a mouse model
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Cytokine levels associated with experimental malaria pathology during Plasmodium berghei ANKA infection in a mouse model

机译：小鼠模型中伯氏疟原虫ANKA感染过程中与实验性疟疾病理相关的细胞因子水平

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Successful recovery from malaria involves striking a balance between counteracting cytokines. The cytokine imbalance contributes to pathological features, but their exact levels have not been elucidated. This study aimed at investigating the role played by circulating cytokines in pathophysiology of cerebral malaria using experimental cerebral malaria (ECM) model by profiling four serum cytokines using cytometric bead array. 72 BALB/c mice were intraperitoneally inoculated with 0.2 ml 1×104?parasitized red blood cells at day 0 and randomized into six groups (six mice/group). The mice were sacrificed at day 4, 6, 8, 11 and 20 post-infection. Significantly higher systemic levels (P<0.05) of interferon-gamma (IFN-γ) were observed between day 8 and 20 post inoculation (p.i), while tumor necrosis factor-alpha (TNF-α) levels were significant at days 4, 8 11, 14 and 20, respectively in BALB/c mice that survived until day 20 post-infection with a higher parasitemia (up to 52.6%±0.8). Significant concentrations (P<0.05) of interleukin-4 (IL-4) were observed between day 4 and 8. IL-5 levels had significant differences at days 11 and 20 p.i. T-cell pathology was revealed by fragmentation of whole genomic DNA during the infection which coincided with elevated IFN-γ and TNF-α responses further accelerating the severity of cerebral malaria (CM). This study has demonstrated the correlation between T-lymphocyte pathology and elevated levels of?T-helper 1 (Th1) cytokines concentrations in the brain and spleen.
机译：从疟疾中成功康复涉及在抵消细胞因子之间取得平衡。细胞因子的失衡有助于病理特征，但其确切水平尚未阐明。这项研究旨在通过使用细胞计数珠阵列分析四种血清细胞因子来研究实验性脑疟疾（ECM）模型中循环细胞因子在脑疟疾病理生理中的作用。在第0天，对72只BALB / c小鼠腹膜内接种0.2ml 1×104？寄生红细胞，随机分为六组（每组六只）。在感染后第4、6、8、11和20天处死小鼠。接种后第8天至第20天观察到全身性干扰素-γ（IFN-γ）水平较高（P <0.05），而肿瘤坏死因子-α（TNF-α）水平在第4、8天显着升高分别在感染后第20天生存并具有较高寄生虫血症（高达52.6％±0.8）的BALB / c小鼠中分别具有11、14和20的特征。在第4天和第8天之间观察到白细胞介素4（IL-4）的显着浓度（P <0.05）。IL-5水平在p.i第11天和第20天有显着差异。 T细胞病理学表现为感染期间整个基因组DNA断裂，这与IFN-γ和TNF-α反应升高同时发生，进一步加剧了脑疟疾（CM）的严重程度。这项研究证明了T淋巴细胞的病理状态与脑和脾脏中的？T-helper 1（Th1）细胞因子浓度升高之间存在相关性。

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