...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of clinical laboratory analysis. >An improved reference method for serum cations measurement by ion chromatography
【24h】

An improved reference method for serum cations measurement by ion chromatography

机译：一种改进的离子色谱法测定血清阳离子的参考方法

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Background To improve the accuracy of the routine methods in laboratory medicine, ion chromatography with a simple sample treatment procedure, which can completely remove the proteins and/or organics in human serum, has been developed for the determination of serum cations. Methods Chromatographic conditions for the separate and simultaneous determination of K, Na, Ca, and Mg were investigated. Furthermore, various factors influencing the mineralization of human serum, such as the selection and amount of oxidant, were also examined systematically and optimized. Results The optimized experimental conditions are as follows: 1.0?mL of serum specimen digested with 2?mL nitric acid (120°C) followed by 2?mL hydrogen peroxide (80°C). The specimens were then redissolved and determined by ion chromatography under the optimum eluent concentration of 32?mmol/L methanesulfonic acids. The measurement accuracy and precision are less than 1.0% for all the analytes by analyzing NIST certified reference materials, IFCC‐RELA specimens and serum specimens. The results were also comparable with the reference values obtained by the inductively coupled plasma mass spectrometry (ICP‐MS), which were found to be in good agreement. Conclusions Ion chromatography with a simple sample treatment procedure for the determination of cations in human serum with high sensitivity and specificity was developed. The proposed method could be recommended as a candidate reference method for the determination of serum cations.
机译：背景技术为了提高实验室医学中常规方法的准确性，已经开发出一种具有简单样品处理程序的离子色谱法，该方法可以完全去除人血清中的蛋白质和/或有机物，用于测定血清阳离子。方法研究了同时测定钾，钠，钙和镁的色谱条件。此外，还系统地研究和优化了影响人血清矿化的各种因素，例如氧化剂的选择和数量。结果优化的实验条件如下：1.0？mL的血清标本先用2？mL的硝酸（120°C）消化，再用2？mL的过氧化氢（80°C）消化。然后将样品重新溶解，并通过离子色谱法在32？mmol / L甲磺酸的最佳洗脱液浓度下进行测定。通过分析NIST认证的参考材料，IFCC-RELA标本和血清标本，所有分析物的测量精度和精度均低于1.0％。结果也与通过电感耦合等离子体质谱（ICP-MS）获得的参考值相当，发现它们具有很好的一致性。结论建立了一种采用简单样品处理方法的离子色谱法，该方法可高灵敏度，高特异性地测定人血清中的阳离子。该方法可以推荐作为确定血清阳离子的参考方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号