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首页> 外文期刊>Journal of clinical laboratory analysis. >An improved reference method for serum cations measurement by ion chromatography
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An improved reference method for serum cations measurement by ion chromatography

机译：离子色谱法测定血清阳离子测量的改进参考方法

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Background To improve the accuracy of the routine methods in laboratory medicine, ion chromatography with a simple sample treatment procedure, which can completely remove the proteins and/or organics in human serum, has been developed for the determination of serum cations. Methods Chromatographic conditions for the separate and simultaneous determination of K, Na, Ca, and Mg were investigated. Furthermore, various factors influencing the mineralization of human serum, such as the selection and amount of oxidant, were also examined systematically and optimized. Results The optimized experimental conditions are as follows: 1.0?mL of serum specimen digested with 2?mL nitric acid (120°C) followed by 2?mL hydrogen peroxide (80°C). The specimens were then redissolved and determined by ion chromatography under the optimum eluent concentration of 32?mmol/L methanesulfonic acids. The measurement accuracy and precision are less than 1.0% for all the analytes by analyzing NIST certified reference materials, IFCC ‐ RELA specimens and serum specimens. The results were also comparable with the reference values obtained by the inductively coupled plasma mass spectrometry ( ICP ‐ MS ), which were found to be in good agreement. Conclusions Ion chromatography with a simple sample treatment procedure for the determination of cations in human serum with high sensitivity and specificity was developed. The proposed method could be recommended as a candidate reference method for the determination of serum cations.
机译：背景技术为了提高实验室药物中常规方法的准确性，具有简单的样品处理程序的离子色谱，可以完全除去人血清中的蛋白质和/或有机物，用于测定血清阳离子。方法研究了用于单独和同时测定K，Na，Ca和Mg的色谱条件。此外，还系统地和优化了影响人血清矿化的各种因素，例如选择和氧化剂的量。结果优化的实验条件如下：1.0？ml用2×ml硝酸（120℃）消化的血清样本，然后用2×mL过氧化氢（80℃）。然后将样品重新溶解并通过离子色谱法在32μmmol/ L甲磺酸的最佳洗脱液浓度下通过离子色谱法测定。通过分析NIST认证的参考资料，IFCC - Rela样本和血清样本，测量精度和精度小于1.0％的所有分析物。结果也与通过感应耦合等离子体质谱（ICP - MS）获得的参考值相当，这被发现与一致一致。结论采用简单样品处理程序的离子色谱，用于测定人血清中具有高敏感性和特异性的阳离子。所提出的方法可以推荐作为用于测定血清阳离子的候选参考方法。

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