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首页> 外文期刊>Journal of Diabetes Science and Technology >Detection of Trace Glucose on the Surface of a Semipermeable Membrane Using a Fluorescently Labeled Glucose-Binding Protein: A Promising Approach to Noninvasive Glucose Monitoring
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Detection of Trace Glucose on the Surface of a Semipermeable Membrane Using a Fluorescently Labeled Glucose-Binding Protein: A Promising Approach to Noninvasive Glucose Monitoring

机译：使用荧光标记的葡萄糖结合蛋白检测半透膜表面的痕量葡萄糖：一种有前途的无创葡萄糖监测方法。

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Background: Our motivation for this study was to develop a noninvasive glucose sensor for low birth weight neonates. We hypothesized that the underdeveloped skin of neonates will allow for the diffusion of glucose to the surface where it can be sampled noninvasively. On further study, we found that measurable amounts of glucose can also be collected on the skin of adults. Method: Cellulose acetate dialysis membrane was used as surrogate for preterm neonatal skin. Glucose on the surface was collected by saline-moistened swabs and analyzed with glucose-binding protein (GBP). The saline-moistened swab was also tested in the neonatal intensive care unit. Saline was directly applied on adult skin and collected for analysis with two methods: GBP and high-performance anion-exchange chromatography (HPAEC). Results: The amount of glucose on the membrane surface was found (1) to accumulate with time but gradually level off, (2) to be proportional to the swab dwell time, and (3) the concentration of the glucose solution on the opposite side of the membrane. The swab, however, failed to absorb glucose on neonatal skin. On direct application of saline onto adult skin, we were able to measure by HPAEC and GBP the amount of glucose collected on the surface. Blood glucose appears to track transdermal glucose levels. Conclusions: We were able to measure trace amounts of glucose on the skin surface that appear to follow blood glucose levels. The present results show modest correlation with blood glucose. Nonetheless, this method may present a noninvasive alternative to tracking glucose trends.
机译：背景：我们进行这项研究的动机是为低出生体重的新生儿开发无创葡萄糖传感器。我们假设新生儿皮肤欠发达，将允许葡萄糖扩散到可无创取样的表面。在进一步的研究中，我们发现成年人皮肤上也可以收集到可测量量的葡萄糖。方法：醋酸纤维素透析膜作为早产儿新生儿皮肤的替代物。用蘸有盐的棉签收集表面的葡萄糖，并用葡萄糖结合蛋白（GBP）进行分析。还在新生儿重症监护室中测试了用盐水浸湿的棉签。将盐水直接涂在成人皮肤上，并用两种方法收集盐进行分析：GBP和高效阴离子交换色谱法（HPAEC）。结果：发现膜表面的葡萄糖量（1）随时间积累但逐渐稳定，（2）与拭子停留时间成正比，（3）另一侧的葡萄糖溶液浓度膜的然而，拭子不能吸收新生儿皮肤上的葡萄糖。将盐水直接施用到成年皮肤上后，我们能够通过HPAEC和GBP来测量表面收集的葡萄糖量。血糖似乎跟踪经皮葡萄糖水平。结论：我们能够测量似乎跟随血糖水平的皮肤表面微量葡萄糖。目前的结果显示与血糖适度相关。但是，该方法可能会提供一种非侵入性的替代方法来跟踪葡萄糖趋势。

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