• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Dentistry >Antimicrobial Activity of Peganum Harmala L. on Streptococcus mutans Compared to 0.2% Chlorhexidine
【24h】

Antimicrobial Activity of Peganum Harmala L. on Streptococcus mutans Compared to 0.2% Chlorhexidine

机译：相对于0.2％洗必泰，百日草对变形链球菌的抗菌活性

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Statement of the Problem: Dental caries is one the most prevalent diseases that affects humans throughout their lives. Streptococcus mutans ( S. mutans ) is recognized as the most important microorganism during tooth cariogenicity. Reducing this germ in oral cavity can reduce the rate of tooth decays in humans. Purpose: The present study compared the antimicrobial activity of ethanolic extract of Peganum harmala L. seeds and 0.2% chlorhexidine on S. mutans . Materials and Method: Agar diffusion technique and micro broth dilution method were employed to test the antimicrobial effects of these two agents on S. mutans . Moreover, the cytotoxicity of ethanolic extract of P. harmala was studied on Vero cells by MTT (thiazolyl blue tetrazolium dye) colorimetric method. The data were analyzed with descriptive methods. Results: Concentrations of 50, 25, and 12.5 mg/mL of the extract made inhibition zones of bacterial growth around the wells; but, lower concentrations could not inhibit the growth of S. mutans . Besides, the antimicrobial effect of 0.2% chlorhexidine was more than 50 mg/mL of the extract. Minimum inhibitory concentration (MIC) of the extract on S. mutans was 1.83±0.6 mg/mL and minimum bactericidal concentration (MBC) was 4.3±1 mg/mL. The MIC and MBC for 0.2% chlorhexidine were reported to be 0.19 mg/mL, and 0.78 mg/mL, respectively. The extract concentrations more than 0.5 mg/mL were toxic and caused more than 50% Vero cell death. Conclusion: Despite the remarkable antimicrobial effects of high concentrations of P. harmala on S. mutans , high cell toxicity of this plant would restrict its in vivo therapeutic use.
机译：问题陈述：龋齿是影响人类一生的最普遍的疾病之一。变形链球菌（变形链球菌）被认为是牙齿致龋期间最重要的微生物。减少口腔中的这种细菌可以减少人类蛀牙的速度。目的：本研究比较了骆驼蓬种子乙醇提取物和0.2％洗必泰对变形链球菌的抗菌活性。材料与方法：采用琼脂扩散技术和微培养液稀释法测试这两种药物对变形链球菌的抗菌作用。此外，通过MTT（噻唑基蓝四唑鎓染料）比色法研究了骆驼藻乙醇提取物对Vero细胞的细胞毒性。用描述性方法分析数据。结果：浓度为50、25和12.5 mg / mL的提取物形成了孔周围细菌生长的抑制区。但是，较低的浓度不能抑制变形链球菌的生长。此外，0.2％洗必泰的抗菌作用大于提取物的50 mg / mL。提取物对变形链球菌的最小抑制浓度（MIC）为1.83±0.6 mg / mL，最小杀菌浓度（MBC）为4.3±1 mg / mL。据报道0.2％洗必泰的MIC和MBC分别为0.19 mg / mL和0.78 mg / mL。提取物浓度超过0.5 mg / mL有毒，并导致超过50％的Vero细胞死亡。结论：尽管高浓度的harm。P. harmala对变形链球菌具有显着的抗菌作用，但该植物的高细胞毒性将限制其体内治疗用途。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号