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首页> 外文期刊>Journal of Dentistry >Determination of Antibacterial Activity of Anacyclus Pyrethrum Extract against Some of the Oral Bacteria: An In Vitro Study
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Determination of Antibacterial Activity of Anacyclus Pyrethrum Extract against Some of the Oral Bacteria: An In Vitro Study

机译：除虫菊除虫菊提取物对某些口腔细菌的抗菌活性测定：一项体外研究

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Statement of Problem: There are more than 500 different bacterial species in the oral cavity which can cause tooth decay and periodontal diseases. Anacyclus Pyrethrum has been used to manage dental and periodontal diseases in traditional Iranian medicine. Purpose: The purpose of this study was to determine the antibacterial activity of Anacyclus Pyrethrum against ?some of the oral bacteria, such as Staphylococcus aureus, Streptococcus mutans , Streptococcus sanguis and Pseudomonas aeruginosa .? Materials and Method: ? At first, The antibacterial effect of serial concentrations (1/10 to 1/100 mg/ml) of methanolic extract of Anacyclus Pyrethrum root were tested by using well assay method on Staphylococcus aureus, Streptococcus mutans, Streptococcus ?sanguis and Pseudomonas aeruginosa .In the second examination, 150-1000 mg/ml concentrations were tested and the agar dilution method, recommended by the Clinical and Laboratory Standards Institute Standards, was used. Then, the lowest concentrations of the extract which inhibited visible growth of organisms on the media plate; Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC), were determined. ??? Results: The inhibition zone was only seen in the 1.10 mg/ml concentration. The diameters were 15 and 12 mm in Staphylococcus aureus and Streptococcus sanguis agar media plate, respectively. In the second examination, the greatest inhibition zones were 27 mm for Staphylococcus aureus and 21 mm for Streptococcus ?sanguis in 1000 mg/ml. There was not any inhibition zone for Streptococcus mutans and Pseudomonas aeruginosa in the concentrations. The MBC was achieved as 800 mg/ml for Staphylococcus aureus and Streptococcus sanguis. Streptococcus mutans and Pseudomonas aeruginosa grew in all the concentrations. Conclusion: The antibacterial effect of Anacyclus Pyrethrum extract against Staphylococcus aureus and Streptococcus sanguis was not significant. Anacyclus Pyrethrum had no antibacterial effect against either Streptococcus mutans or Pseudomonas aeruginosa .
机译：问题陈述：口腔中有500多种不同的细菌种类，它们可能导致蛀牙和牙周疾病。 Anacyclus除虫菊已被用于管理伊朗传统医学中的牙齿和牙周疾病。目的：本研究的目的是确定除虫菊除虫菊酯对某些口腔细菌的抗菌活性，如金黄色葡萄球菌，变形链球菌，血链球菌和铜绿假单胞菌。材料与方法：首先，采用良好的分析方法，测定了一系列浓度（1/10至1/100 mg / ml）的除虫菊除虫菊根甲醇提取物对金黄色葡萄球菌，变形链球菌，桑氏链球菌和铜绿假单胞菌的抗菌作用。第二次检查，测试浓度为150-1000 mg / ml，并使用临床和实验室标准协会标准推荐的琼脂稀释方法。然后，抑制培养基板上微生物可见生长的最低提取物浓度;确定了最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。 ???结果：仅在1.10 mg / ml的浓度下可见抑制区。在金黄色葡萄球菌和血链球菌琼脂培养基平板中的直径分别为15和12mm。在第二次检查中，以1000 mg / ml的浓度，金黄色葡萄球菌的最大抑制区为27 mm，血链球菌的最大抑制区为21 mm。在该浓度下，对变形链球菌和铜绿假单胞菌没有任何抑制区。对于金黄色葡萄球菌和血链球菌，MBC达到800 mg / ml。变形链球菌和铜绿假单胞菌均在所有浓度下生长。结论：除虫菊除虫菊提取物对金黄色葡萄球菌和血链球菌的抑菌作用不明显。 Anacyclus除虫菊对变形链球菌或铜绿假单胞菌均无抗菌作用。

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