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首页> 外文期刊>Journal of Epithelial Biology & Pharmacology >Role for the Bacterial Signal Indole in Promoting Epithelial Cell Barrier Function
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Role for the Bacterial Signal Indole in Promoting Epithelial Cell Barrier Function

机译：细菌信号吲哚在促进上皮细胞屏障功能中的作用

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Interkingdom (IK) signaling, in which pathogenic bacteria recognize human hormones in the gastrointestinaltract to initiate infection, has recently emerged as an important contributor to gastrointestinal tract infections. While amajority of studies have primarily focused on the effect of host molecules on pathogens, recent work from our labhas demonstrated that human intestinal epithelial cells can also recognize bacterial signaling molecules. We demonstratedthat indole, secreted to ~ 500 μM by commensal Escherichia coli, increased expression of tight junction proteins andgenes involved in mucin production in HCT-8 intestinal epithelial cells, thus enhancing their barrier properties. Theseresults were consistent with an observed increased in trans-epithelial resistance on exposure to indole over 24 h. Sinceindole is structurally similar to melatonin, and melatonin is a hormone that regulates the dark-cycle in the eye, wealso investigated role of indole on human retinal pigment epithelium (RPE) cells. Our data show that indole increasestrans-epithelial resistance of RPE cells, and attenuated their proliferation and migration. Our results suggest a beneficialrole for indole in the treatment of eye diseases, and are discussed in detail in this article.
机译：病态细菌识别胃肠道中的人类激素以引发感染的Interkingdom（IK）信号最近已成为胃肠道感染的重要因素。尽管大量研究主要集中在宿主分子对病原体的作用上，但我们实验室的最新研究表明，人肠上皮细胞也可以识别细菌信号分子。我们证明了由共生大肠埃希菌分泌至〜500μM的吲哚增加了HCT-8肠上皮细胞中粘蛋白产生所涉及的紧密连接蛋白和基因的表达，从而增强了它们的屏障特性。这些结果与观察到的吲哚经24小时的跨上皮抵抗力增加一致。由于吲哚在结构上与褪黑激素相似，并且褪黑激素是一种调节眼部黑暗周期的激素，因此我们还研究了吲哚对人视网膜色素上皮细胞（RPE）的作用。我们的数据表明，吲哚可增加RPE细胞的跨上皮耐药性，并减弱其增殖和迁移。我们的研究结果提示了吲哚在治疗眼部疾病中的有益作用，并在本文中进行了详细讨论。

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