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Behavior of Native Microbial Populations of WPC-34 and WPC-80 Whey Protein Stored at Different Temperatures

机译:WPC-34和WPC-80乳清蛋白在不同温度下存储的天然微生物种群的行为

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Whey protein (WPC34 and 80) has been used as food ingredients and as a base for making biodegradable product. However, there is limited information on the behavior of native microflora associated with these products. The objective of this study was to estimate classes of native microflora of WPC34 and WPC80 using different agar media and then investigate how storage temperatures (5, 10, 15, 22 and 30°C) for 7 days would affect survival and growth of each class of the bacteria estimated. Immediately after receiving WPC34 and WPC80 from the manufacturer the initial populations of aerobic mesophilic bacteria, coliform, yeast and mold, lactic acid bacteria including lipolytic bacteria were enumerated and bioluminescent Adenosine Triphosphate (ATP) assay was used to estimate ATP level corresponding to the overall microbial populations associated with WPC34 and WPC80 products. Total microbial populations in WPC34 and WPC80 determined immediately and after storage for 7 days averaged 6.8 log and 7.1 log CFU/g, respectively and the corresponding ATP values associated with the total microbial populations averaged 62 and 73 RLU, respectively. Class of microorganism estimated from WPC80 averaged 2.8 log CFU/g for aerobic mesophilic bacteria, below detection ( 0.05) changes in total microbial populations of WPC34 and WPC80 and bioluminescent ATP assay can effectively be used to estimate total microbial populations in WPC34 and WPC80 products.
机译:乳清蛋白(WPC34和80)已被用作食品成分和制造可生物降解产品的基础。但是,有关与这些产品相关的天然菌群行为的信息有限。这项研究的目的是使用不同的琼脂培养基评估WPC34和WPC80的天然菌群种类,然后研究7天的储存温度(5、10、15、22和30°C)如何影响每个种类的存活和生长。估计的细菌数。从制造商处获得WPC34和WPC80后,立即计算出了好氧嗜温细菌,大肠菌,酵母和霉菌的初始种群,其中包括脂解性细菌的乳酸菌被枚举,并使用生物发光三磷酸腺苷(ATP)分析法来估计对应于整个微生物的ATP水平与WPC34和WPC80产品相关的人群。立即和在储存7天后确定的WPC34和WPC80中的总微生物种群平均分别为6.8 log CFU / g和7.1 log CFU / g,与总微生物种群相关的相应ATP值分别为62和73 RLU。从WPC80估计的好氧嗜温细菌的微生物类别平均为2.8 log CFU / g,低于WPC34和WPC80的总微生物种群的检测值(0.05)变化以下,并且生物发光ATP分析可有效地用于估计WPC34和WPC80产品的总微生物种群。

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