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Bioreduction of Cr (VI) by Heavy Metal Resistant Pseudomonas Species

机译:耐重金属假单胞菌对铬(VI)的生物还原

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Chromium (VI) contamination has accelerated due to rapid industrialization worldwide. Aim of this study was to check the bacterial species for their tolerance towards multiple metals, antibiotics and further check whether these bacteria are reducing Cr (VI). Bacterial strains were isolated from the industrial area situated at Lagos-Abeokuta Road, Ile Ise Awo, Abeokuta, Ogun State, Nigeria. All of the isolates showed tolerance to lead, zinc and chromium (VI). Bacterial species also showed tolerance towards antibiotics, 100% of strains were resistant to ampicillin, cotrimoxazole, colistin and gentamycin, 66.6% to nitrofurantoin and nalidixic acid, 83.3% were resistant to streptomycin whereas, 50% were resistant to tetracycline. Among all the strains, only two strains Pseudomonas strain PH2 and PH4 were chosen for chromium (VI) reduction as these strains showed maximum tolerance towards chromium (VI). Maximum reduction (60%) of chromium (VI) was observed at pH 6 by Pseudomonas spp. PH2, which was followed by pH 5 (50%) whereas, pH9 showed least reduction of 12.5%. Similarly, Pseudomonas spp. PH4 also reduced chromium considerably at pH 6 (60%), pH 5 (50%), pH 8 (37.5%) and at pH 9 (12.5%) respectively, at a concentration of 100 μg Cr mL-1 after 120 h of incubation. The Pseudomonas species PH2 reduced chromium (VI) at concentration of 50 μg Cr mL-1 (92%), 100 μg Cr mL-1 (70%) and 150 μg Cr mL-1 (50%), respectively at a pH of 6. Similarly, Pseudomonas species PH4 reduced chromium (VI) by 95% at 50 μg Cr mL-1, 70% at 100 μg Cr mL-1 and 55% at 150 μg Cr mL-1 at a pH of 6. Due to above properties strains could therefore, be used as bioremediators of metals in soils contaminated with heavy metals and can also increase the yield of various crops under heavy metal contamination.
机译:由于世界范围内的快速工业化,铬(VI)污染已经加速。这项研究的目的是检查细菌对多种金属,抗生素的耐受性,并进一步检查这些细菌是否正在还原Cr(VI)。从位于尼日利亚奥贡州阿贝库塔的Ise Ise Awo拉各斯阿贝库塔路的工业区分离出细菌菌株。所有分离株均显示出对铅,锌和铬(VI)的耐受性。细菌种类也显示出对抗生素的耐受性,100%的菌株对氨苄青霉素,cotrimoxazole,粘菌素和庆大霉素具有耐药性,对硝基呋喃妥因和萘啶酸的耐药率为66.6%,对链霉素的耐药率为83.3%,而对四环素的耐药率为50%。在所有菌株中,仅选择了两个假单胞菌菌株PH2和PH4还原铬(VI),因为这些菌株显示出对铬(VI)的最大耐受性。假单胞菌(Pseudomonas spp)在pH 6时,铬(VI)的最大还原量(60%)。 PH2,其后是pH 5(50%),而pH9的降低最少为12.5%。同样,假单胞菌属。在pH 6(60%),pH 5(50%),pH 8(37.5%)和pH 9(12.5%)下,PH4还分别以100μgCr mL -1 <的浓度显着还原铬。 / SUP>孵育120小时后。假单胞菌PH2在50μgCr mL -1 (92%),100μgCr mL -1 (70%)和150μg的浓度下还原铬(VI) Cr mL -1 (50%)分别在pH值为6的情况下发生。类似地,假单胞菌种PH4在50μgCr mL -1 下将铬(VI)还原了95%。 ,在pH为6时,在100μgCr mL -1 中为70%,在150μgCr mL -1 中为55%。由于上述特性,因此可以使用菌株作为重金属污染土壤中金属的生物修复剂,还可以增加重金属污染下各种农作物的产量。

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