• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of genetics >A deep sequencing analysis of transcriptomes and the development of EST-SSR markers in mungbean (Vigna radiata)
【24h】

A deep sequencing analysis of transcriptomes and the development of EST-SSR markers in mungbean (Vigna radiata)

机译：绿豆（Vigna radiata）转录组的深层测序分析和EST-SSR标记的发展

获取原文
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Mungbean (Vigna radiata L. Wilczek) is one of the most important leguminous food crops in Asia. We employed Illumina paired-end sequencing to analyse transcriptomes of three different mungbean genotypes. A total of 38.3a€“39.8 million paired-end reads with 73 bp lengths were generated. The pooled reads from the three libraries were assembled into 56,471 transcripts. Following a cluster analysis, 43,293 unigenes were obtained with an average length of 739 bp and N50 length of 1176 bp. Of the unigenes, 34,903 (80.6%) had significant similarity to known proteins in the NCBI nonredundant protein database (Nr), while 21,450 (58.4%) had BLAST hits in the Swiss-Prot database (E-value 10a??a?μ). Further, 1245 differential expression genes were detected among three mungbean genotypes. In addition, we identified 3788 expressed sequence tag-simple sequence repeat (EST-SSR) motifs that could be used as potential molecular markers. Among 320 tested loci, 310 (96.5%) yielded amplification products, and 151 (47.0%) exhibited polymorphisms among six mungbean accessions. These transcriptome data and mungbean EST-SSRs could serve as a valuable resource for novel gene discovery and the marker-assisted selective breeding of this specie
机译：绿豆（Vigna radiata L. Wilczek）是亚洲最重要的豆科粮食作物之一。我们采用Illumina的配对末端测序来分析三种不同绿豆基因型的转录组。总共产生了长度为73 bp的38.3a€3,980万配对配对读段。来自三个文库的合并读段被组装成56,471个转录本。经过聚类分析，获得了43293个单基因，平均长度为739 bp，N50长度为1176 bp。在单基因中，有34,903（80.6％）与NCBI非冗余蛋白质数据库（Nr）中的已知蛋白质具有显着相似性，而在Swiss-Prot数据库中有21,450（58.4％）的BLAST命中（E值<10a ?? a ?？ μ）。此外，在三种绿豆基因型中检测到1245个差异表达基因。此外，我们确定了3788个表达的序列标签-简单序列重复（EST-SSR）模体，可以用作潜在的分子标记。在320个测试位点中，有310个（96.5％）产生了扩增产物，而151个（47.0％）在六个绿豆种之间表现出多态性。这些转录组数据和绿豆EST-SSRs可作为该物种新基因发现和标记辅助选育的宝贵资源

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号